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      Evaluation of antimicrobial efficacy of Aloe vera and its effectiveness in decontaminating gutta percha cones

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          Abstract

          Aim:

          The aim of this study was to evaluate the antimicrobial efficacy of Aloe vera and to determine its effectiveness in decontaminating gutta percha cones.

          Materials and Methods:

          A concentrated extract of Aloe vera was used to check for the antimicrobial efficacy using the agar well diffusion method. Presence of zones’ of diffusion was identified against three common GP contaminants namely, E.coli, E.faecalis and Staph. aureus. New GP Cones, freshly taken out of the packet were then decontaminated for 1minute using Aloe vera gel and then placed in thioglycolate broth to check for the presence of turbidity.

          Results:

          The zones of inhibition on the agar plate were measured as 24mm,21mm and 24mm respectively. The broth remained clear even after 48 hours of incubation.

          Conclusion:

          We conclude that Aloe vera is indeed effective as a GP decontaminant and it holds a promising future as a medium for storage of GP cones.

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          Most cited references17

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          Disinfection of gutta-percha cones with chlorhexidine and sodium hypochlorite.

          To evaluate the effectiveness of chlorhexidine (CHX) or sodium hypochlorite (NaOCl) in disinfecting gutta-percha cones, to verify contamination of gutta-percha cones in their boxes, and to identify microorganisms after intentional contamination by handling cones. Gutta-percha cones contaminated in vitro with several microorganisms were left in contact with tested disinfecting solutions for different times, sterility of storage boxes was evaluated by immersing cones in broth medium, and the microorganisms most frequently found in handling cones were identified using biochemical tests. CHX was not effective in eliminating Bacillus subtilis spores on gutta-percha cones after 72 h of contact with the disinfecting substance. 5.25% NaOCl eliminated spores from gutta-percha after 1 min of disinfection. The cones evaluated from their boxes did not show contamination in 94.5% of the cases. The microbial genus most frequently found after intentional contamination with gloves was Staphylococcus. 5.25% NaOCl is an effective agent for a rapid disinfection of gutta-percha cones.
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            Analysis of phytochemical constituents and antimicrobial activities of Aloe vera L. against clinical pathogens

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              A historic review of endodontics, 1689-1963, part 1.

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                Author and article information

                Journal
                J Conserv Dent
                J Conserv Dent
                JCD
                Journal of Conservative Dentistry : JCD
                Medknow Publications & Media Pvt Ltd (India )
                0972-0707
                0974-5203
                Jul-Sep 2012
                : 15
                : 3
                : 246-248
                Affiliations
                [1]Department of Conservative dentistry and Endodontics, Mahatma Gandhi Post Gradute Institute of Dental Sciences, (Govt.of Puducherry Institution), Puducherry, India
                Author notes
                Address for correspondence: Dr. Prakash P Athiban, Department of Conservative dentistry and Endodontics, Mahatma Gandhi Post Gradute Institute of Dental Sciences, (Govt. of Puducherry Institution), Indira Nagar, Gorimedu, Puducherry – 605 006, India E-mail: prakash4bds@ 123456gmail.com
                Article
                JCD-15-246
                10.4103/0972-0707.97949
                3410334
                22876011
                b9ead3cf-b4f9-40cd-bc94-d821a97677f6
                Copyright: © Journal of Conservative Dentistry

                This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

                History
                : 26 January 2012
                : 10 March 2012
                : 13 March 2012
                Categories
                Original Article

                Dentistry
                decontamination,aloe vera,gutta percha cones
                Dentistry
                decontamination, aloe vera, gutta percha cones

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