+1 Recommend
1 collections
      • Record: found
      • Abstract: found
      • Article: found

      1H NMR Quantification of DHA and EPA in Fish Oil

      Read this article at

          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.


          Fish oil is a popular nutritional product consumed in China. The beneficial effects of fish oil have been attributed to docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA). Hence, it is crucial to develop a rapid and precise method to determine the contents of EPA and DHA in fish oil. In this study, a rapid and accurate proton nuclear magnetic resonance method for the quantification of DHA and EPA was developed. Dimethyl terephthalate was selected as an internal standard, and the signals at 2.391 ppm for DHA and at 1.697 ppm for EPA were chosen for the quantification. Validation of the method was performed in terms of specificity, precision, and stability. The results indicated that the method was precise and in line with the China Food and Drug Administration guidance. The method has been successfully applied to characterize fish oil capsules obtained from four pharmaceutical companies. This study indicated that the rapid 1H NMR method is suitable for the quality control of fish oil.

          Related collections

          Author and article information

          Journal of Ocean University of China
          Science Press and Springer (China )
          02 September 2020
          01 October 2020
          : 19
          : 5
          : 1193-1197
          1School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China
          2Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology, Qingdao 266003, China
          3Key Laboratory of Glycoscience & Glycotechnology of Shandong Province, Qingdao 266003, China
          4Key Laboratory of Marine Drugs, Ministry of Education of China, Qingdao 266003, China
          Author notes
          *Corresponding authors: LV Zhihua, E-mail: lvzhihua@ ; YU Mingming, E-mail: yumingming@

          # The two authors contributed equally to the work.

          Copyright © Ocean University of China, Science Press and Springer-Verlag GmbH Germany 2020.

          The copyright to this article, including any graphic elements therein (e.g. illustrations, charts, moving images), is hereby assigned for good and valuable consideration to the editorial office of Journal of Ocean University of China, Science Press and Springer effective if and when the article is accepted for publication and to the extent assignable if assignability is restricted for by applicable law or regulations (e.g. for U.S. government or crown employees).

          Self URI (journal-page):


          Comment on this article