1 H NMR Quantification of DHA and EPA in Fish Oil

Author(s): LV Jinxiao ¹ ^, ^# , WANG Cong ¹ ^, ² ^, ³ ^, ⁴ ^, ^# , ZHANG Xiuli ¹ ^, ² ^, ³ ^, ⁴ , LV Zhihua ¹ ^, ² ^, ³ ^, ⁴ ^, , YU Mingming ¹ ^, ² ^, ³ ^, ⁴ ^,

Publication date (Electronic): 02 September 2020

Journal: Journal of Ocean University of China

Publisher: Science Press and Springer

Keywords: 1H NMR , EPA, DHA, fish oil, quality control

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Abstract

Fish oil is a popular nutritional product consumed in China. The beneficial effects of fish oil have been attributed to docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA). Hence, it is crucial to develop a rapid and precise method to determine the contents of EPA and DHA in fish oil. In this study, a rapid and accurate proton nuclear magnetic resonance method for the quantification of DHA and EPA was developed. Dimethyl terephthalate was selected as an internal standard, and the signals at 2.391 ppm for DHA and at 1.697 ppm for EPA were chosen for the quantification. Validation of the method was performed in terms of specificity, precision, and stability. The results indicated that the method was precise and in line with the China Food and Drug Administration guidance. The method has been successfully applied to characterize fish oil capsules obtained from four pharmaceutical companies. This study indicated that the rapid ¹H NMR method is suitable for the quality control of fish oil.

Author and article information

Journal

Journal ID (publisher-id): JOUC

Title: Journal of Ocean University of China

Publisher: Science Press and Springer (China )

ISSN (Electronic): 1672-5182

Publication date (Electronic): 02 September 2020

Publication date (Print): 01 October 2020

Volume: 19

Issue: 5

Pages: 1193-1197

Affiliations

[1] ¹School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China

[2] ²Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology, Qingdao 266003, China

[3] ³Key Laboratory of Glycoscience & Glycotechnology of Shandong Province, Qingdao 266003, China

[4] ⁴Key Laboratory of Marine Drugs, Ministry of Education of China, Qingdao 266003, China

Author notes

*Corresponding authors: LV Zhihua, E-mail: lvzhihua@ 123456ouc.edu.cn ; YU Mingming, E-mail: yumingming@ 123456ouc.edu.cn

# The two authors contributed equally to the work.

Article

Publisher ID: s11802-020-4395-3

DOI: 10.1007/s11802-020-4395-3

SO-VID: ba06f664-b2b1-4591-b574-656257688670

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