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      Accurate determination of microbial diversity from 454 pyrosequencing data.

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          Abstract

          We present an algorithm, PyroNoise, that clusters the flowgrams of 454 pyrosequencing reads using a distance measure that models sequencing noise. This infers the true sequences in a collection of amplicons. We pyrosequenced a known mixture of microbial 16S rDNA sequences extracted from a lake and found that without noise reduction the number of operational taxonomic units is overestimated but using PyroNoise it can be accurately calculated.

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          Author and article information

          Journal
          Nat Methods
          Nature methods
          Springer Science and Business Media LLC
          1548-7105
          1548-7091
          Sep 2009
          : 6
          : 9
          Affiliations
          [1 ] Department of Civil Engineering, Rankine Building, University of Glasgow, Glasgow, UK. quince@civil.gla.ac.uk
          Article
          nmeth.1361
          10.1038/nmeth.1361
          19668203
          ba132bea-d557-47ec-a108-ae4cd007e20b
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