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High throughput analysis at microscale: performance of ionKey/MS with Xevo G2-XS QTof under rapid gradient conditions

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      In this paper, high throughput analysis with 3 minute, rapid gradient conditions is described using the ionKey/MS™ System with an integrated ACQUITY UPLC® M-Class System and the Xevo® G2-XS QTof Mass Spectrometer. Extensive testing of representative small molecules and a peptide shows that the system is well-tolerated and exhibits excellent reproducibility and linear response. The iKey™ HSS T3 Separation Device used is robust, withstanding ~2200 injections of prepared human plasma with excellent peak shape and system pressure profile. A 99% solvent savings was realized when compared with an analytical system using a 2.1 mm column with flow rate ranging from 0.6 mL/min to 1.5 mL/min. These data, coupled with examples from the literature, illustrate that the ionKey/MS System with Xevo G2-XS QTof can be used as a full service platform for high throughput analysis and high sensitivity analysis to support all phases of drug discovery and development.

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      Journal of Applied Bioanalysis
      J Appl Bioanal
      Betasciencepress Publishing
      October 15 2015
      October 15 2015
      : 1
      : 4
      : 128-135
      © 2015

      This work is licensed under a Creative Commons Attribution 3.0 Unported License. To view a copy of this license, visit

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      General life sciences, Chemistry, Analytical chemistry, Life sciences


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