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      Subsídios para o estudo histopatológico das lesões ungueais Translated title: Base for the histopathological study of nail lesions

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          Abstract

          FUNDAMENTOS: Os autores apresentam ampla revisão sobre os fundamentos da biópsia da unidade ungueal, fornecendo bases teóricas sobre anatomia, histologia e histopatologia. Abordam, detalhadamente, as indicações e os objetivos da biópsia da unidade ungueal e as várias técnicas disponíveis. OBJETIVOS: Definir os locais adequados da biópsia do aparelho ungueal para as diferentes doenças da unha, comparar duas formas de amaciamento da lâmina ungueal, uma utilizando KOH a 20%, outra, solução aquosa de Tween 40 a 10%, e estabelecer uma rotina para o processamento do material de biópsia. MÉTODOS: O grupo de estudo constou de 11 pacientes com doença ungueal, que procuraram o ambulatório de Dermatologia do HUPE/UERJ para esclarecimento diagnóstico. Foi realizado o total de 12 biópsias. RESULTADOS: Não houve diferença na dificuldade técnica, em incluir ou cortar o material, consoante ao método de amaciamento utilizado, nem prejuízo citológico ou histológico ao exame histopatológico. CONCLUSÕES: Os autores concluíram que biópsia ungueal é procedimento seguro e eficaz para esclarecer o diagnóstico das doenças ungueais quando o exame clínico ou outras técnicas falharam. Também é importante, para quem deseja realizar o procedimento, o conhecimento adequado da anatomia dessa região.

          Translated abstract

          BACKGROUND: In this paper a broad review of nail biopsy is presented, emphasizing its anatomy, embryology, histology and histopathology. The indication and purpose of nail biopsy as well as the techniques available are reviewed in detail. OBJECTIVE: Define the appropriate site for nail biopsy according to nail disorder, compare two different methods of nail specimen softening: soaking in 20% potassium hydroxide and in 10% aqueous solution of Tween 40 and establish a routine for the nail biopsy procedure. METHODS: Our group studied eleven patients with nail disease who presented at the Dermatology Department in Pedro Ernesto University Hospital for a definite diagnosis. Twelve biopsies were performed. RESULTS: There were no differences in the softness achieved with the two methods and none harmed the cytologic and histologic analysis. CONCLUSIONS: The authors concluded that nail biopsy is a safe and useful procedure for nail diseases when clinical and laboratory methods fail to reach a diagnosis. A complete and thorough understanding of the nail unit anatomy is necessary to perform the procedure

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          Anatomia: Estudo regional do corpo humano

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            Nail is produced by the normal nail bed: a controversy resolved

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              Diagnostic techniques for confirming onychomycosis.

              Other nail diseases may mimic a fungal infection. Therefore the diagnosis of onychomycosis requires an assessment of both clinical and laboratory features. Onychomycosis is comprised of four distinct types, each of which requires a different technique to obtain a nail specimen. A potassium hydroxide (KOH) preparation may be used to confirm the presence of fungi. However, a fungal culture is required to identify the specific genus and species of the pathogen. It may be necessary to perform histopathologic analysis of the nail unit in cases in which the clinical appearance suggests the presence of a fungal nail infection, but the KOH preparation and culture are negative.
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                Author and article information

                Contributors
                Role: ND
                Role: ND
                Role: ND
                Journal
                abd
                Anais Brasileiros de Dermatologia
                An. Bras. Dermatol.
                Sociedade Brasileira de Dermatologia (Rio de Janeiro )
                1806-4841
                February 2003
                : 78
                : 1
                : 49-61
                Affiliations
                [1 ] Universidade do Estado do Rio de Janeiro Brazil
                Article
                S0365-05962003000100005
                10.1590/S0365-05962003000100005
                bb47618e-8eec-4bcd-875d-128260a28748

                http://creativecommons.org/licenses/by/4.0/

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                SciELO Brazil

                Self URI (journal page): http://www.scielo.br/scielo.php?script=sci_serial&pid=0365-0596&lng=en
                Categories
                DERMATOLOGY

                Dermatology
                biopsy,nail diseases,pathology,biópsia,doenças da unha,patologia
                Dermatology
                biopsy, nail diseases, pathology, biópsia, doenças da unha, patologia

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