0
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      Evaluation of the efficacy of disinfectants against Puumala hantavirus by real-time RT-PCR

      brief-report

      Read this article at

      ScienceOpenPublisherPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Puumala virus, a hantavirus belonging to the Bunyaviridae family, causes a human disease known as nephropathia epidemica, a mild form of hemorrhagic fever with renal syndrome. The implementation of effective decontamination procedures is critical in hantavirus research to minimize the risk of personnel exposure. This study investigated the efficacy of Clidox ®, Dettol ®, ethanol, Halamid-d ®, peracetic acid, sodium hypochloride and Virkon ®S for inactivating Puumala virus. A real-time quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) was used to quantify Puumala virus before and after treatment with these products. Inactivation of Puumala virus was effective after 10 min with all products except ethanol. Inactivation with absolute ethanol was effective only after 30 min. Using the qRT-PCR method, this study has shown that the commercially available products Clidox ®, Halamid-d ® and Virkon ®S in particular represent a rapid and safe way to decontaminate surfaces with possible Puumala virus contamination. These products can be used in solutions of 1–2%, with contact times greater than 10 min, for inactivating effectively Puumala virus.

          Related collections

          Most cited references28

          • Record: found
          • Abstract: found
          • Article: not found

          Development of one-step, real-time, quantitative reverse transcriptase PCR assays for absolute quantitation of human coronaviruses OC43 and 229E.

          The clinical significance of human coronaviruses in more severe respiratory illnesses has recently been shown to be higher than was previously assumed. Rapid and reliable diagnosis of human coronavirus infections therefore becomes indispensable in a routine clinical setting. In this study, we present a very sensitive and specific TaqMan-based, real-time quantitative reverse transcriptase PCR (qRT-PCR) for the rapid detection and quantitation of human coronaviruses (HCoVs) OC43 and 229E. Absolute viral load measurement in clinical samples was achieved through the construction of in-house HCoV OC43 and 229E cRNA standards for the generation of a standard curve. The HCoV OC43 assay allows quantitation over a range from 20 to 2 x 10(8) RNA copies per reaction mixture (5 microl RNA extract). When this is extrapolated to clinical samples, this corresponds to a detection range of 10(3) to 10(10) viral genome equivalents per ml. By using the HCoV 229E qRT-PCR assay, viral RNA copies ranging from 200 to 2 x 10(9) per reaction mixture can be detected, which corresponds to 10(4) to 10(11) viral genome equivalents per ml sample. A total of 100 respiratory samples screened for the presence of HCoVs OC43 and 229E by using conventional RT-PCR were assessed in parallel by the qRT-PCR assays. By use of the real-time qRT-PCR techniques, the detection rate of HCoVs OC43 and 229E increased from 2.0% to 3.1% and from 0.3% to 2.5%, respectively. The real-time qRT-PCR assays described here allow the rapid, specific, and sensitive laboratory detection and quantitation of human coronaviruses OC43 and 229E.
            Bookmark
            • Record: found
            • Abstract: found
            • Article: not found

            Antigenic and genetic properties of viruses linked to hemorrhagic fever with renal syndrome.

            Hemorrhagic fever with renal syndrome (HFRS) comprises a variety of clinically similar diseases of viral etiology that are endemic to and sporadically epidemic throughout the Eurasian continent and Japan. Although HFRS has not been reported in North America, viruses that are antigenically similar to HFRS agents were recently isolated from rodents in the United States. Examination and comparison of eight representative isolates from endemic disease areas and from regions with no known associated HFRS indicate that these viruses represent a new and unique group that constitutes a separate genus in the Bunyaviridae family of animal viruses.
              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              Hantavirus pulmonary syndrome in Panama: identification of novel hantaviruses and their likely reservoirs.

              Hantavirus pulmonary syndrome (HPS), a severe respiratory disease with high mortality caused by rodent-borne hantaviruses, has previously been identified in the United States and Canada as well as central and southern South America. In late 1999 and early 2000, an outbreak of acute illness compatible with HPS was reported in Los Santos, Panama, with the death of 3 of the 12 (25%) suspected cases. Hantavirus-specific antibodies were detected in patient sera, and virus RNA was detected by reverse transcriptase-polymerase chain reaction. Sequence analysis of virus genome N-, G1-, and G2-encoding fragments showed this to be a novel hantavirus, Choclo virus. Serologic and virus genetic analyses of rodents trapped in the area showed Oligoryzomys fulvescens to be the likely reservoir for the HPS-associated Choclo virus. In addition, Zygodontomys brevicauda rodents were shown to harbor another genetically unique hantavirus, Calabazo virus. Copyright 2000 Academic Press.
                Bookmark

                Author and article information

                Contributors
                Journal
                J Virol Methods
                J. Virol. Methods
                Journal of Virological Methods
                Elsevier B.V.
                0166-0934
                1879-0984
                22 December 2006
                April 2007
                22 December 2006
                : 141
                : 1
                : 111-115
                Affiliations
                [a ]Laboratory of Clinical and Epidemiological Virology, Rega Institute, Minderbroedersstraat 10, B3000 Leuven, Belgium
                [b ]Laboratory of Immunobiology, Rega Institute, Minderbroedersstraat 10, B3000 Leuven, Belgium
                Author notes
                [* ]Corresponding author at: Laboratory of Clinical and Epidemiological Virology, Department of Microbiology & Immunology, Rega Institute, Minderbroedersstraat 10, B3000 Leuven, Belgium. Tel.: +32 16 332166; fax: +32 16 332131. pmaes3@ 123456uz.kuleuven.be
                Article
                S0166-0934(06)00424-1
                10.1016/j.jviromet.2006.11.037
                7185759
                17188760
                bb7403b7-bcaa-4584-98ad-67bcde6b1f0d
                Copyright © 2006 Elsevier B.V. All rights reserved.

                Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.

                History
                : 21 September 2006
                : 20 November 2006
                : 27 November 2006
                Categories
                Article

                Microbiology & Virology
                decontamination,taqman pcr,quantitative pcr,real-time pcr,puumala virus
                Microbiology & Virology
                decontamination, taqman pcr, quantitative pcr, real-time pcr, puumala virus

                Comments

                Comment on this article