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      The Irr and RirA Proteins Participate in a Complex Regulatory Circuit and Act in Concert To Modulate Bacterioferritin Expression in Ensifer meliloti 1021

      , , , ,

      Applied and Environmental Microbiology

      American Society for Microbiology

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          ABSTRACT

          In this work we found that the bfr gene of the rhizobial species Ensifer meliloti , encoding a bacterioferritin iron storage protein, is involved in iron homeostasis and the oxidative stress response. This gene is located downstream of and overlapping the smc03787 open reading frame (ORF). No well-predicted RirA or Irr boxes were found in the region immediately upstream of the bfr gene although two presumptive RirA boxes and one presumptive Irr box were present in the putative promoter of smc03787 . We demonstrate that bfr gene expression is enhanced under iron-sufficient conditions and that Irr and RirA modulate this expression. The pattern of bfr gene expression as well as the response to Irr and RirA is inversely correlated to that of smc03787 . Moreover, our results suggest that the small RNA SmelC759 participates in RirA- and Irr-mediated regulation of bfr expression and that additional unknown factors are involved in iron-dependent regulation.

          IMPORTANCE E. meliloti belongs to the Alphaproteobacteria , a group of bacteria that includes several species able to associate with eukaryotic hosts, from mammals to plants, in a symbiotic or pathogenic manner. Regulation of iron homeostasis in this group of bacteria differs from that found in the well-studied Gammaproteobacteria . In this work we analyzed the effect of rirA and irr mutations on bfr gene expression. We demonstrate the effect of an irr mutation on iron homeostasis in this bacterial genus. Moreover, results obtained indicate a complex regulatory circuit where multiple regulators, including RirA, Irr, the small RNA SmelC759, and still unknown factors, act in concert to balance bfr gene expression.

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          Author and article information

          Journal
          Applied and Environmental Microbiology
          Appl. Environ. Microbiol.
          American Society for Microbiology
          0099-2240
          1098-5336
          August 01 2017
          August 15 2017
          August 15 2017
          June 16 2017
          : 83
          : 16
          Article
          10.1128/AEM.00895-17
          5541210
          28625986
          © 2017
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