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      Spatial regulation of cytoplasmic snRNP assembly at the cellular level

      Journal of Experimental Botany
      Oxford University Press
      cajal bodies, confocal microscopy, in situ hybridization, larix, p bodies, plant cell, sm proteins, snrna, splicing.

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          Abstract

          Highlight: Under physiological conditions, it was shown that the same cell model may establish two distinct spatial manners of cytoplasmic snRNP assembly.

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          Most cited references58

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          Small nucleolar RNAs: an abundant group of noncoding RNAs with diverse cellular functions.

          Small nucleolar RNAs represent an abundant, evolutionarily ancient group of noncoding RNAs which possess impressively diverse functions ranging from 2'-O-methylation and pseudouridylation of various classes of RNAs, through nucleolytic processing of rRNAs to the synthesis of telomeric DNA.
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            Cajal body-specific small nuclear RNAs: a novel class of 2'-O-methylation and pseudouridylation guide RNAs.

            Cajal (coiled) bodies are conserved subnuclear organelles that are present in the nucleoplasm of both animal and plant cells. Although Cajal bodies were first described nearly 100 years ago, their function has remained largely speculative. Here, we describe a novel class of human small nuclear RNAs that localize specifically to Cajal bodies. The small Cajal body-specific RNAs (scaRNAs) are predicted or have already been demonstrated to function as guide RNAs in site-specific synthesis of 2'-O-ribose-methylated nucleotides and pseudouridines in the RNA polymerase II-transcribed U1, U2, U4 and U5 spliceosomal small nuclear RNAs (snRNAs). Our results provide strong support for the idea that the Cajal body, this mysterious nuclear organelle, provides the cellular locale for post-transcriptional modification of spliceosomal snRNAs.
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              Spliceosomal UsnRNP biogenesis, structure and function.

              Significant advances have been made in elucidating the biogenesis pathway and three-dimensional structure of the UsnRNPs, the building blocks of the spliceosome. U2 and U4/U6*U5 tri-snRNPs functionally associate with the pre-mRNA at an earlier stage of spliceosome assembly than previously thought, and additional evidence supporting UsnRNA-mediated catalysis of pre-mRNA splicing has been presented.
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                Author and article information

                Journal
                26320237
                4765780
                10.1093/jxb/erv399
                http://creativecommons.org/licenses/by/3.0

                Plant science & Botany
                cajal bodies,confocal microscopy,in situ hybridization,larix,p bodies,plant cell,sm proteins,snrna,splicing.

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