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      Analysis and sequencing of h6hmRNA, last enzyme in the tropane alkaloids pathway from anthers and hairy root cultures of Brugmansia candida (Solanaceae)

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          Abstract

          Brugmansia candida (Solanaceae) is a native tree distributed across South-American and produces the pharmacologically- important group of tropane alkaloids including scopolamine. This biocompound is synthesised from hyoscyamine by action of Hyoscyamine 6-β hydroxylase (H6H, EC 1.14.11.11) at the end of the tropane alkaloid pathway. Here are reported the tissue and organ-specific expression of h6hmRNA by RT-PCR analyses and the isolation, cloning and sequencing of the cDNA obtained from B. candida anthers and hairy root transformed cultures. Bioinformatic analysis of the nucleotide sequence revealed an uninterrupted ORF of 1038 bp and the predicted aminoacid sequence could be 344 aminoacid long. A database search showed that this sequence has high homology (97% identity) to Hyoscyamus niger H6H protein (Genbank accession number AAA33387.1).

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          Most cited references 8

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          New genes in alkaloid metabolism and transport.

          The biosynthetic pathway of plant alkaloids is composed of several distinct enzymes of varying substrate specificities. Homology-based cloning of candidate genes and their subsequent functional testing in heterologous expression systems are accelerating the pace at which the gene catalogues of alkaloid biosynthesis are expanding. Availability of diverse genes involved in the biosynthesis, catabolism, transport, and regulation of pharmaceutically important alkaloids should steadily advance our molecular understanding of alkaloid biology and will enable us to devise more rational strategies for metabolic engineering.
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            Functional expression of tropinone reductase I (trI) and hyoscyamine-6β-hydroxylase (h6h) from Hyoscyamus niger in Nicotiana tabacum

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              Tropane Alkaloid Production in Transformed Root Cultures ofBrugmansia candida

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                Author and article information

                Contributors
                Role: ND
                Role: ND
                Role: ND
                Journal
                ejb
                Electronic Journal of Biotechnology
                Electron. J. Biotechnol.
                Pontificia Universidad Católica de Valparaíso and CONICYT (Valparaíso )
                0717-3458
                June 2006
                : 9
                : 3
                : 0
                Affiliations
                [1 ] Universidad de Buenos Aires Argentina
                [2 ] Fundación Pablo Cassará Argentina
                S0717-34582006000300004
                10.4067/S0717-34582006000300004

                http://creativecommons.org/licenses/by/4.0/

                Product
                Product Information: SciELO Chile
                Categories
                BIOTECHNOLOGY & APPLIED MICROBIOLOGY

                Biotechnology

                transformation, sequence analysis, scopolamine, hyoscyamine, hairy roots, H6H

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