In the literature there are different opinions about serum prolactin levels after the menopause and in women using hormone replacement therapy. We report serum prolactin levels of 165 women after surgical or natural menopause who received different kinds of hormone replacement therapies over 2 years and 6 months. Surgically or naturally menopausal women showed no significant differences in serum prolactin levels. After starting hormone replacement therapy, the serum prolactin levels decreased. During subsequent treatment cycles, serum prolactin showed varying levels within the reference limits. Women using hormone replacement therapy developed no new manifestation of hyperprolactinemia.