Electrotaxis of lung cancer cells in ordered three-dimensional scaffolds

There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

Abstract

In this paper, we report a new method to incorporate 3D scaffold with electrotaxis measurement in the microfluidic device. The electrotactic response of lung cancer cells in the 3D foam scaffolds which resemble the in vivo pulmonary alveoli may give more insight on cellular behaviors in vivo. The 3D scaffold consists of ordered arrays of uniform spherical pores in gelatin. We found that cell morphology in the 3D scaffold was different from that in 2D substrate. Next, we applied a direct current electric field (EF) of 338 mV/mm through the scaffold for the study of cells' migration within. We measured the migration directedness and speed of different lung cancer cell lines, CL1-0, CL1-5, and A549, and compared with those examined in 2D gelatin-coated and bare substrates. The migration direction is the same for all conditions but there are clear differences in cell morphology, directedness, and migration speed under EF. Our results demonstrate cell migration under EF is different in 2D and 3D environments and possibly due to different cell morphology and/or substrate stiffness.

Related collections

Most cited references 69

Record: found
Abstract: found
Article: not found

Tissue cells feel and respond to the stiffness of their substrate.

Dennis E. Discher, Paul A. Janmey, Yu-Li Wang (2005)

Normal tissue cells are generally not viable when suspended in a fluid and are therefore said to be anchorage dependent. Such cells must adhere to a solid, but a solid can be as rigid as glass or softer than a baby's skin. The behavior of some cells on soft materials is characteristic of important phenotypes; for example, cell growth on soft agar gels is used to identify cancer cells. However, an understanding of how tissue cells-including fibroblasts, myocytes, neurons, and other cell types-sense matrix stiffness is just emerging with quantitative studies of cells adhering to gels (or to other cells) with which elasticity can be tuned to approximate that of tissues. Key roles in molecular pathways are played by adhesion complexes and the actinmyosin cytoskeleton, whose contractile forces are transmitted through transcellular structures. The feedback of local matrix stiffness on cell state likely has important implications for development, differentiation, disease, and regeneration.

0 comments Cited 507 times – based on 0 reviews      Review now

Bookmark

Record: found
Abstract: found
Article: not found

Fused deposition modeling of novel scaffold architectures for tissue engineering applications.

Iwan Zein, Dietmar W. Hutmacher, Kim Cheng Tan … (2002)

Fused deposition modeling, a rapid prototyping technology, was used to produce novel scaffolds with honeycomb-like pattern, fully interconnected channel network, and controllable porosity and channel size. A bioresorbable polymer poly(epsilon-caprolactone) (PCL) was developed as a filament modeling material to produce porous scaffolds, made of layers of directionally aligned microfilaments, using this computer-controlled extrusion and deposition process. The PCL scaffolds were produced with a range of channel size 160-700 microm, filament diameter 260-370 microm and porosity 48-77%, and regular geometrical honeycomb pores, depending on the processing parameters. The scaffolds of different porosity also exhibited a pattern of compressive stress-strain behavior characteristic of porous solids under such loading. The compressive stiffness ranged from 4 to 77 MPa, yield strength from 0.4 to 3.6 MPa and yield strain from 4% to 28%. Analysis of the measured data shows a high correlation between the scaffold porosity and the compressive properties based on a power-law relationship.

0 comments Cited 168 times – based on 0 reviews      Review now

Bookmark

Record: found
Abstract: found
Article: not found

Electrospinning polyaniline-contained gelatin nanofibers for tissue engineering applications.

Hua Li, J MacDiarmid, Kuo-Yen Wei … (2006)

Polyaniline (PANi), a conductive polymer, was blended with a natural protein, gelatin, and co-electrospun into nanofibers to investigate the potential application of such a blend as conductive scaffold for tissue engineering purposes. Electrospun PANi-contained gelatin fibers were characterized using scanning electron microscopy (SEM), electrical conductivity measurement, mechanical tensile testing, and differential scanning calorimetry (DSC). SEM analysis of the blend fibers containing less than 3% PANi in total weight, revealed uniform fibers with no evidence for phase segregation, as also confirmed by DSC. Our data indicate that with increasing the amount of PANi (from 0 to approximately 5%w/w), the average fiber size was reduced from 803+/-121 nm to 61+/-13 nm (p<0.01) and the tensile modulus increased from 499+/-207 MPa to 1384+/-105 MPa (p<0.05). The results of the DSC study further strengthen our notion that the doping of gelatin with a few % PANi leads to an alteration of the physicochemical properties of gelatin. To test the usefulness of PANi-gelatin blends as a fibrous matrix for supporting cell growth, H9c2 rat cardiac myoblast cells were cultured on fiber-coated glass cover slips. Cell cultures were evaluated in terms of cell proliferation and morphology. Our results indicate that all PANi-gelatin blend fibers supported H9c2 cell attachment and proliferation to a similar degree as the control tissue culture-treated plastic (TCP) and smooth glass substrates. Depending on the concentrations of PANi, the cells initially displayed different morphologies on the fibrous substrates, but after 1 week all cultures reached confluence of similar densities and morphology. Taken together these results suggest that PANi-gelatin blend nanofibers might provide a novel conductive material well suited as biocompatible scaffolds for tissue engineering.

0 comments Cited 164 times – based on 0 reviews      Review now

Bookmark

All references

Author and article information

Journal

Title: Biomicrofluidics

Abbreviated Title: Biomicrofluidics

Publisher: AIP Publishing

ISSN (Electronic): 1932-1058

Publication date Created: March 2012

Publication date (Print): March 2012

Volume: 6

Issue: 1

Page: 014102

Affiliations

[1 ]Research Center for Applied Sciences, Academia Sinica, Taipei City 11529, Taiwan

[2 ]Institute of Biophotonics, National Yang-Ming University, Taipei City 11221, Taiwan

[3 ]Institute of Physics, Academia Sinica, Taipei City 11529, Taiwan

[4 ]Department of Mechanical and Mechantronic Engineering, National Taiwan Ocean University, Keelung 20224, Taiwan

Article

DOI: 10.1063/1.3671399

PMC ID: 3267495

PubMed ID: 22288000

SO-VID: bc2f7004-8840-4510-80ac-a2a2c87b948b

History

Data availability:

Comments

Comment on this article

scite_

Cited by 19

See all cited by

Most referenced authors 1,238

See all reference authors

- Version 1
- Version 1

Electrotaxis of lung cancer cells in ordered three-dimensional scaffolds

Read this article at

Abstract

Related collections

Gamma Delta T cells

Most cited references 69

Tissue cells feel and respond to the stiffness of their substrate.

Fused deposition modeling of novel scaffold architectures for tissue engineering applications.

Electrospinning polyaniline-contained gelatin nanofibers for tissue engineering applications.

Author and article information

Journal

Affiliations

Article

History

Comments

Comment on this article

Similar content 3,280

Cited by 19

Most referenced authors 1,238