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      The extracellular matrix as a cell survival factor.

      1 , 1 , 1
      Molecular Biology of the Cell
      American Society for Cell Biology (ASCB)

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          Abstract

          Programmed cell death (PCD) or apoptosis is a naturally occurring cell suicide pathway induced in a variety of cell types. In many cases, PCD is induced by the withdrawal of specific hormones or growth factors that function as survival factors. In this study, we have investigated the potential role of the extracellular matrix (ECM) as a cell survival factor. Our results indicate that in the absence of any ECM interactions, human endothelial cells rapidly undergo PCD, as determined by cell morphology, nuclei fragmentation, DNA degradation, protein cross-linking, and the expression of the PCD-specific gene TRPM-2. PCD was blocked by plating cells on an immobilized integrin beta 1 antibody but not by antibodies to either the class I histocompatibility antigen (HLA) or vascular cell adhesion molecule-1 (VCAM-1), suggesting that integrin-mediated signals were required for maintaining cell viability. Treatment of the cells in suspension with the tyrosine phosphatase inhibitor sodium orthovanadate also blocked PCD. When other cell types were examined, some, but not all, underwent rapid cell death when deprived of adhesion to the ECM. These results suggest that in addition to regulating cell growth and differentiation, the ECM also functions as a survival factor for many cell types.

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          Author and article information

          Journal
          Molecular Biology of the Cell
          MBoC
          American Society for Cell Biology (ASCB)
          1059-1524
          1939-4586
          September 1993
          September 1993
          : 4
          : 9
          : 953-961
          Affiliations
          [1 ]Scripps Research Institute, Committee on Vascular Biology, La Jolla, California 92037.
          Article
          10.1091/mbc.4.9.953
          275725
          8257797
          bc42f0ea-68ac-454f-8d08-a639793decdc
          © 1993
          History

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