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      Unbiased estimation of total β-cell number and mean β-cell volume in rodent pancreas

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      APMIS

      Wiley

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          Most cited references 6

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          The efficiency of systematic sampling in stereology and its prediction.

          The superior efficiency of systematic sampling at all levels in stereological studies is emphasized and various commonly used ways of implementing it are briefly described. Summarizing recent theoretical and experimental studies a set of very simple estimators of efficiency are presented and illustrated with a variety of biological examples. In particular, a nomogram for predicting the necessary number of points when performing point counting is provided. The very efficient and simple unbiased estimator of the volume of an arbitrary object based on Cavalieri's principle is dealt with in some detail. The efficiency of the systematic fractionating of an object is also illustrated.
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            The unbiased estimation of number and sizes of arbitrary particles using the disector.

             D Sterio (1984)
            A three-dimensional counting rule and its integral test system, the disector, for obtaining unbiased estimates of the number of arbitrary particles in a specimen is presented. Used in combination with ordinary and recently developed stereological methods unbiased estimates of various mean particle sizes and the variance of particle volume are obtainable on sets of two parallel sections with a known separation. The same principle allows the unbiased estimation of the distribution of individual particle volumes in sets of serial sections.
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              Stereology of arbitrary particles*

               H. Gundersen (1986)
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                Author and article information

                Journal
                APM
                APMIS
                Wiley
                09034641
                16000463
                March 1999
                March 1999
                : 107
                : 7-12
                : 791-799
                10.1111/j.1699-0463.1999.tb01474.x
                © 1999

                http://doi.wiley.com/10.1002/tdm_license_1.1

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