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      A RT-PCR method for selective amplification and phenotypic characterization of all three serotypes of Sabin-related polioviruses from viral mixtures

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          Abstract

          Outbreaks caused by vaccine-derived polioviruses are challenging the final eradication of paralytic poliomyelitis. Therefore, the surveillance of the acute flaccid paralysis cases based on poliovirus isolation and characterization remains an essential activity. Due to the use of trivalent oral poliovirus vaccine (OPV), mixtures containing more than one serotype of Sabin-related polioviruses are frequently isolated from clinical samples. Because each poliovirus isolate needs to be individually analyzed, we designed polymerase chain reaction primers that can selectively distinguish and amplify a genomic segment of the three Sabin-related poliovirus serotypes present in mixtures, thus, optimizing the diagnosis and providing prompt information to support epidemiologic actions.

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          Most cited references 9

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          Vaccine-derived poliovirus (VDPV): Impact on poliomyelitis eradication.

          The live attenuated strains used in the oral poliovirus (OPV) have been the main tool in the WHO polio eradication programme. However, these strains replicate in the human gut and are excreted for several weeks after immunisation. During this period, the attenuating mutations in the vaccine strains can rapidly revert. This may, in rare cases, cause vaccine-associated paralytic poliomyelitis (VAPP) in vaccinees or result in transmissible and neurovirulent circulating vaccine-derived poliovirus (cVDPV) strains. Outbreaks of poliomyelitis caused by VDPV have recently occurred in communities with long-term incomplete immunisation coverage. Hypogammaglobulinaemic vaccinees can chronically excrete immunodeficient VDPV (iVDPV) for several decades. As long as OPV is used, cVDPV and iVDPV pose a risk of causing poliomyelitis in unprotected individuals and threaten the goal of poliovirus eradication. VDPV cannot arise from the inactivated poliovirus vaccine (IPV), but financial and logistical barriers to replace OPV with IPV remain.
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            Polio Laboratory Manual

            (2004)
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              From emergence to eradication: the epidemiology of poliomyelitis deconstructed

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                Author and article information

                Affiliations
                [1 ] Fundação Oswaldo Cruz Brazil
                Contributors
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Journal
                mioc
                Memórias do Instituto Oswaldo Cruz
                Mem. Inst. Oswaldo Cruz
                Instituto Oswaldo Cruz, Ministério da Saúde (Rio de Janeiro )
                1678-8060
                August 2012
                : 107
                : 5
                : 698-701
                S0074-02762012000500022 10.1590/S0074-02762012000500022

                http://creativecommons.org/licenses/by/4.0/

                Product
                Product Information: SciELO Brazil
                Categories
                PARASITOLOGY
                TROPICAL MEDICINE

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