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      Performance characteristics of nested polymerase chain reaction vs real-time polymerase chain reaction methods for detecting Mycobacterium tuberculosis complex in paraffin-embedded human tissues.

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          Abstract

          Nucleic acid amplification tests on formalin-fixed, paraffin-embedded (FFPE) tissue specimens enable Mycobacterium tuberculosis complex (MTB) detection and rapid tuberculosis diagnosis in the absence of microbiologic culture tests. We aimed to evaluate the efficacy of different polymerase chain reaction (PCR) methods for detecting Mycobacterium species in FFPE tissues.

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          Author and article information

          Journal
          Am. J. Clin. Pathol.
          American journal of clinical pathology
          American Society for Clinical Pathology (ASCP)
          1943-7722
          0002-9173
          Sep 2014
          : 142
          : 3
          Affiliations
          [1 ] From the Department of Pathology, Seoul National University Bundang Hospital, Seongnam, Gyeonggido, Republic of Korea; Department of Pathology, Kyungpook National University School of Medicine, Jung-gu, Daegu, Republic of Korea;
          [2 ] From the Department of Pathology, Seoul National University Bundang Hospital, Seongnam, Gyeonggido, Republic of Korea;
          [3 ] Department of Laboratory Medicine, Seoul National University Bundang Hospital, Seongnam, Gyeonggido, Republic of Korea; and.
          [4 ] From the Department of Pathology, Seoul National University Bundang Hospital, Seongnam, Gyeonggido, Republic of Korea; Departments of Pathology and.
          [5 ] Department of Laboratory Medicine, Seoul National University Bundang Hospital, Seongnam, Gyeonggido, Republic of Korea; and Laboratory Medicine, Seoul National University College of Medicine, Jongnogu, Seoul, Republic of Korea. m91w95pf@snu.ac.kr.
          Article
          142/3/384
          10.1309/AJCP2QZRH4ZNPRDD
          25125630
          bccf3379-21d6-445b-88f3-6f5d7615ba69
          History

          Nested PCR,paraffin-embedded tissues,Mycobacterium tuberculosis,Formalin-fixed,Real-time PCR

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