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      Age-Related Reciprocal Modulation of lnterleukin-1βand lnterleukin-1 Receptors in the Mouse Brain-Endocrine-Immune Axis

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          Abstract

          The cytokine interleukin-1 (IL-1) alters a variety of immune, central nervous system and neuroendocrine activities characteristic of an integrator of the brain-endocrine-immune response to stress. In an attempt to define the regulation of IL-1 and IL-1 receptors in the mouse brain-endocrine-immune axis during maturation, we measured tissue levels of IL-lβusing an ELISA and iodine- 125-labeled recombinant human interleukin-1α([<sup>125</sup>I]IL-lα) binding in hippocampus, pituitary, testis and spleen following intraperitoneal injection of saline or the bacterial endotoxin, lipopolysaccharide (LPS) in 3- and 24-week-old C57BL/6 mice. Basal IL-lβlevels were detectable in all the tissues examined. Basal levels of IL-1β in the hippocampus, spleen and testis in 24-week-old mice were significantly higher than those in 3-week-old mice. [<sup>125</sup>I]IL-lαbinding was detectable in all the mouse tissues examined and [<sup>125</sup>I]IL-1αbinding levels in the pituitary, spleen and testis in 3-week-old mice were significantly higher than those in 24-week-old mice. To further evaluate the modulation of IL-1 receptors in aging, we measured [<sup>125</sup>I]IL-1αbinding in 2-, 5-, 10-, 18-, and 24-month-old mice. [<sup>125</sup>I]IL-1αbinding in testis in 24-month-old mice was higher than the other groups; [<sup>125</sup>I]IL-1αbinding in the hippocampus and spleen was unchanged during these periods. Dramatic increases in IL-lβconcentrations were observed at 2 h after LPS injection in the pituitary, spleen, testis and plasma in both 3- and 24-week-old mice groups. In contrast, IL-lβlevels in the hippocampus increased in response to LPS injection only in 24-week-old mice. [<sup>125</sup>I]IL-1αbinding in hippocampus was significantly decreased in 24- but not in 3-week-old mice after LPS injection. [<sup>125</sup>I]IL-1αbinding in the spleen and testis were significantly decreased in both age groups following LPS administration. These data demonstrate differential regulation of IL-1 and its receptors in the brain-endocrine-immune axis during maturation and in response to endotoxin challenge.

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          Author and article information

          Journal
          NIM
          Neuroimmunomodulation
          10.1159/issn.1021-7401
          Neuroimmunomodulation
          S. Karger AG
          1021-7401
          1423-0216
          1996
          1996
          19 March 1997
          : 3
          : 4
          : 205-212
          Affiliations
          aSecond Department of Internal Medicine, Kochi Medical School, Kochi, Japan; bNeurocrine Biosciences, San Diego, Calif., USA
          Article
          97272 Neuroimmunomodulation 1996;3:205–212
          10.1159/000097272
          9094441
          © 1996 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          Page count
          Pages: 8
          Categories
          Original Paper

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