In this article we report two new records of Trachycephalus mambaiensis for the state of Minas Gerais and present a map which represents the known distribution of this species. The record of T. mambaiensis from municipalities of Bonito de Minas and Januária, state of Minas Gerais represents a distribution extension of 190 km southern from the type locality, at the municipality of Mambaí, state of Goiás.