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      Using Intravascular Autoradiography for an Estimation of Proliferative Activity of Rat Mesenteric Microvessel Endothelial Cells during the First Month of Postnatal Development

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          Background/Methods: We applied a novel method for studying endothelial cells (EC) by using autoradiography of cells labeled by <sup>3</sup>H-thymidine: photo emulsion was administered into the vascular bed. In the flat transparent organ, this method allows to compare the mitotic activity of endothelium (MAE) depending on vessel diameter and animal age. Results: The number of the labeled ECs in animals was found to be almost equal in all vessels at the same age. The amount of labeled vessels and the density of the labeled nuclei increase towards near-capillary vessels of a diameter ≤10 µm. With age, ÐœÐE decreases in all categories of vessels. However, in 12-day-old animals, ÐœÐE temporarily increases. It was noted that high ÐœÐE in the bed of these rats precedes or coincides with the period of accelerated weight gain of the digestive system supplied by an increase in organ blood flow. Conclusions: By using the endothelial autoradiography method that we developed, we obtained the following evidence: (1) the number of ECs in the synthetic phase of the mitotic cycle in mesenterial vessels is approximately identical in animals of the same age, and (2) this number decreases with age, except during the period of accelerated organ growth, when MAE increases.

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          The ultrastructure of mammalian arterioles and precapillary sphincters.

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            Ultrastructure of mammalian venous capillaries, venules, and small collecting veins.

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              Mitotic activity of the endothelium of mesenteric microvessels of various diameters in wistar rats


                Author and article information

                J Vasc Res
                Journal of Vascular Research
                S. Karger AG
                August 2007
                30 May 2007
                : 44
                : 5
                : 403-409
                aState Research Institute of Physiology, Laboratory of Microcirculation, Siberian Branch of the Russian Academy of Medical Sciences and bState Pedagogical University, Chair of Anatomy, Physiology and Valeology, Novosibirsk, Russian Federation
                103285 J Vasc Res 2007;44:403–409
                © 2007 S. Karger AG, Basel

                Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

                Page count
                Figures: 3, Tables: 3, References: 12, Pages: 7
                New Methods in Vascular Research


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