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      Proteomic approaches and their application to plant gravitropism.

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          Abstract

          Proteomics is a powerful technique that allows researchers a window into how an organism responds to a mutation, a specific environment, or at a distinct point during development by quantifying relative protein abundance and posttranslational modifications. Here, we describe methods for the proteomic analysis of Arabidopsis thaliana tissue. Extraction protocols are provided for isolation of soluble, plasma membrane, and tonoplast proteins. In addition, basic analysis and quality metrics for MS/MS data are discussed. The protocols outlined have the potential to unlock new avenues of research that are not possible through basic genetics or transcriptomic approaches. By combining proteomic information with known gene regulatory patterns, researchers can gain a complete picture of how molecular pathways, such as those required for gravitropism, are initiated, regulated, and terminated.

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          Author and article information

          Journal
          Methods Mol. Biol.
          Methods in molecular biology (Clifton, N.J.)
          Springer Nature America, Inc
          1940-6029
          1064-3745
          2015
          : 1309
          Affiliations
          [1 ] Department of Environmental and Plant Biology, Ohio University, Athens, OH, USA.
          Article
          10.1007/978-1-4939-2697-8_10
          25981772
          bd3b03a0-e06d-4f05-9f3e-720a8bc207a0
          History

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