A new navigational mechanism mediated by ant ocelli

Desert ants, Cataglyphis, navigate in their vast desert habitat by path integration. They continuously integrate directions steered (as determined by their celestial compass) and distances traveled, gauged by as-yet-unknown mechanisms. Here we test the hypothesis that navigating ants measure distances traveled by using some kind of step integrator, or "step counter." We manipulated the lengths of the legs and, hence, the stride lengths, in freely walking ants. Animals with elongated ("stilts") or shortened legs ("stumps") take larger or shorter strides, respectively, and concomitantly misgauge travel distance. Travel distance is overestimated by experimental animals walking on stilts and underestimated by animals walking on stumps.

As textbook knowledge has it, bees and ants use polarized skylight as a backup cue whenever the main compass cue, the sun, is obscured by clouds. Here we show, by employing a unique experimental paradigm, that the celestial compass system of desert ants, Cataglyphis, relies predominantly on polarized skylight. If ants experience only parts of the polarization pattern during training but the full pattern in a subsequent test situation, they systematically deviate from their true homeward courses, with the systematics depending on what parts of the skylight patterns have been presented during training. This "signature" of the polarization compass remains unaltered, even if the ants can simultaneously experience the sun, which, if presented alone, enables the ants to select their true homeward courses. Information provided by direct sunlight and polarized skylight is picked up by different parts of the ant's compound eyes and is channeled into two rather separate systems of navigation.