We present the first list of beetle species from an urban area in the northeast Andes of Colombia, based on a review of specimens deposited in the insect collection of the Museo de Historia Natural “Luis Gonzalo Andrade” of the Universidad Pedagógica y Tecnológica de Colombia and from sporadic collections from various points around the city of Tunja, Boyacá. We recorded 34 species, 30 genera, 17 subfamilies, and 12 families of beetles. Of these, 10 new records for the department of Boyacá and two new species records for Colombia are reported. In addition, we recorded the type of urban habitat for the individuals of 24 species collected: 11 species in gray areas (roads, sidewalks, houses, buildings) and eight in green areas (parks, pastures, plantations). Our results form a baseline for further studies on biodiversity in urban ecosystems in the Eastern Andes of Colombia.