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      Crecimiento de Listeria monocytogenes en atún ahumado empacado al vacío Translated title: Growth of Listeria monocytogenes in smoked tuna vacuum packaged

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          Abstract

          El pescado con frecuencia se contamina con bacterias patógenas como Listeria monocytogenes, las cuales son capaces de crecer en productos frescos y congelados, y su predominio en pescado ahumado es elevado. Este estudio plantea determinar el crecimiento de L. monocytogenes en un producto ahumado empacado al vacío, elaborado a partir de atún. Las muestras obtenidas de una empresa atunera fueron trasladadas al Laboratorio de Tecnología de Alimentos del INIA-Sucre/Nueva Esparta, donde se les realizaron los análisis para determinar grado de frescura de la materia prima y procesamiento del producto. El atún fue descabezado, eviscerado, lavado y cortado en trozos de aproximadamente 300 g. Se prepararon dos submuestras y se colocaron en salmuera de 8% y 10% por 20 minutos, se inocularon y se ahumaron (40°C y 50°C) por seis horas, se empacaron al vacío y se almacenaron en refrigeración. El inóculo se preparó con cepas certificadas a partir del patrón estándar de Mac Farland 0.4. El crecimiento de L. monocytogenes fue monitoreado cada 7 días, utilizando agar PALCAM. Los resultados promedios de pH (5,74), NBVT (20,85 mg/100g), coliformes (1,1 x 10³ NMP/g) y mesófilos (2,7 x 10(5) UFC/g) realizados a la materia prima, demuestran que el pescado estaba en buenas condiciones para el ensayo y que los valores estuvieron dentro de los límites establecidos. Los resultados sobre el crecimiento de L. monocytogenes durante refrigeración demostraron que el ahumado tuvo efecto inhibidor en la población inicial de L. monocytogenes, comenzando a observarse un ascenso después de los siete días de elaborados los productos

          Translated abstract

          Processed fish frequently gets contaminated with pathogenous bacteria like Listeria monocytogenes, which can grow in fresh or frozen products, with a high prevalence in smoked fish. The objective of this study is to determine growth of L. monocytogenes in a smoked tuna vacuum packed. Samples were obtained from a tuna processing plant and brought to Food Technology Laboratory INIA-Sucre/Nueva Esparta, where freshness analyses were performed on the fresh and the processed products. An inoculum was prepared with certified Mac Farland 0.4 strains. The fish were beheaded, gutted, washed and cut in pieces about 300 g. Two subsamples were prepared which were placed in 8% and 10% brine by 20 min. Then they were inoculated and smoked (40°C and 50°C) by six hours. Afterwards, they were vacuum packed and refrigerated. Growth of L. monocytogenes was monitored every 7 days, using agar PALCAM. Average values of pH (5.74), BTVN (20.85 mg/100g), coliforms (1.1 x 10³ MPN/g) and mesophils (2.7 x 10(5) CFU/g) from the raw material showed that it was in good condition for the essay, since they were under established limits. The results of L. monocytogenes growth during refrigeration showed that smoking had an inhibitory effect of the initial population, but an increase was observed after seven days of processing

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          Most cited references23

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              Growth ofListeria monocytogenesat refrigeration temperatures

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                Author and article information

                Journal
                zt
                Zootecnia Tropical
                Zootecnia Trop.
                Instituto Nacional de Investigaciones Agricolas INIA, Maracay, Venezuela. (Maracay, Aragua, Venezuela )
                0798-7269
                April 2005
                : 23
                : 2
                : 171-181
                Affiliations
                [02] Cumaná Estado Sucre orgnameInstituto Nacional de Investigaciones Agrícola orgdiv1Postgrado de Biología Aplicada orgdiv2CIAE-Sucre/Nueva Esparta Venezuela
                [01] Cumana Estado Sucre orgnameNúcleo Sucre orgdiv1Postgrado de Biología Aplicada Venezuela berthafiguera@ 123456yahoo.com
                Article
                S0798-72692005000200006 S0798-7269(05)02300206
                bd674355-941b-4ee4-ada2-f12a131aac83

                http://creativecommons.org/licenses/by/4.0/

                History
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 23, Pages: 11
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                SciELO Venezuela

                Categories
                Artículos Científicos

                Listeria monocytogenes,smoking tuna,contamination,atún ahumado,contaminación

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