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      [Antibacterial and antifungal effects of Agkistrodon halys Pallas: purification of its antibacterial protein--LAO].

      Shi yan sheng wu xue bao

      Agkistrodon, Amino Acid Oxidoreductases, isolation & purification, metabolism, Animals, Anti-Infective Agents, pharmacology, Crotalid Venoms, enzymology, Escherichia coli, drug effects, Fungi, L-Amino Acid Oxidase

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          Abstract

          This paper reports the venom from Agkistrodon halys Pallas have inhibitory activity against fungi and E. coli by tested in a disc diffusion assay. An antibacterial component--LAO from the venom were purified to homogeneous. It had not only antibacterial effect, but L-amino acid oxidase activity. And its enzymatic specific activity was 808 U/mg. The venom had at least 3 antibacterial components (I, II, III) as determined by acid polyacrilamide gel electrophoresis, LAO is the antibaterial components II.

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          12549069

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