Platelet-derived growth factor (PDGF) is an essential signaling molecule for wound
healing and tissue repair. This study was aimed at evaluating the effect of PDGF on
the proliferation of temporomandibular joint (TMJ) disc-derived cells and extracellular
matrix synthesis. The number of cultured cells were counted by COULTER Z1. The assay
for collagen synthesis was performed using a sircol soluble collagen assay. Hyaluronic
acid (HA) synthesis was analyzed by a high performance liquid chromatography. The
expression of collagens, matrix metalloproteinases (MMPs), and the tissue inhibitors
of metalloproteinases (TIMPs) were examined using SYBR Green in terms of the RNA levels.
PDGF treatment significantly (P < .01) increased the proliferation rate of the disc-derived
cells as compared with the controls when the dose was 5 ng/ mL or greater. Treatment
with more than 5 ng/mL PDGF resulted in an amount of collagen synthesis significantly
(P < .01) higher than the controls. HA synthesis was maximal with 5 ng/mL PDGF treatment.
Quantitative real-time polymerase chain reaction analyses showed that treatment with
5 ng/mL of PDGF-BB upregulated the mitochondrial RNA levels of type I and II collagens,
MMPs, and TIMPs within 6 hours. It is concluded that PDGF, if its concentration is
optimal, enhanced proliferation and matrix synthesis of TMJ disc-derived cells, indicating
that PDGF may be effective for use in tissue engineering of the TMJ disc.