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      In vitro characterization of grass carp (Ctenopharyngodon idella) IL-26 in regulating inflammatory factors.

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          Abstract

          Interleukin 26 (IL-26) gene has been identified in human, amphibian and teleost but not in rodents. It is well accepted that IL-26 was a crucial member of IL-10 family which acts as a pro-inflammatory cytokine in human. However, the role of IL-26 in regulating inflammation in lower vertebrates including teleost has not been defined yet. In the present study, grass carp IL-26 (gcIL-26) coding sequence was isolated and identified. Its chromosomal synteny was also analyzed, showing that gcIL-26 gene is flanked by IL-22 and IFN-γ genes with the same transcriptional orientation as seen in human, amphibian and zebrafish. Given that zebrafish and grass carp IL-26 shared relatively low amino acid identities with human IL-26, the functional roles of fish IL-26 are indispensable to be elucidated. Accordingly, recombinant gcIL-26 (rgcIL-26) was prepared by using Pichia pastoris expression system, and it was found to be partially glycosylated. Using grass carp head kidney leucocytes as cell model, rgcIL-26 displayed the bioactivity to stimulate the mRNA expression of some pro-inflammatory cytokines including IL-8, IL-1β and IL-6, while inhibit mRNA expression of an anti-inflammatory cytokine, IL-10. Moreover, rgcIL-26 also up-regulated inos expression and NO production in grass carp monocytes/macrophages, strengthening its pro-inflammatory properties in fish. Those results collectively demonstrated the functional role of IL-26 in regulating inflammatory response in fish.

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          Author and article information

          Journal
          Fish Shellfish Immunol.
          Fish & shellfish immunology
          Elsevier BV
          1095-9947
          1050-4648
          May 08 2017
          : 66
          Affiliations
          [1 ] Center for Informational Biology, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, People's Republic of China.
          [2 ] Center for Informational Biology, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, People's Republic of China. Electronic address: wangxyhku@uestc.edu.cn.
          Article
          S1050-4648(17)30264-4
          10.1016/j.fsi.2017.05.024
          28495510
          bd9e2240-56d1-4702-984a-d224955770a8
          History

          Functional identification,Gene locus,Grass carp,IL-26,Pro-inflammatory property

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