Evidence for Field-Evolved Resistance of Striacosta albicosta (Lepidoptera: Noctuidae) to Cry1F Bacillus thuringiensis Protein and Transgenic Corn Hybrids in Ontario, Canada

Abstract

<p class="first" id="d2676618e98">Western bean cutworm, Striacosta albicosta (Smith) (Lepidoptera: Noctuidae), is a pest of corn (Zea mays L.) that has recently expanded its range into Ontario, Canada. Control of S. albicosta damage to corn hybrids containing event TC1507-expressing Cry1F Bacillus thuringiensis protein alone or pyramided with event MON 89034 expressing Cry1A.105 and Cry2Ab2 Bt proteins was tested in 2011-2015 in Ontario in small- and large-scale field plots with natural infestation. In 2011, significantly lower incidence and severity of kernel damage was sustained by Cry1F × Cry1A.105 + Cry2Ab2 corn compared with a non-Bt near-isogenic hybrid. However, from 2012 to 2015, there was no difference in incidence or severity of damage comparing non-Bt hybrids with Cry1F hybrids alone or pyramided with Cry1A.105 and Cry2Ab2 planted as a pure stand or with an integrated refuge (95% Bt: 5% non-Bt seeds). In 2015, neonate larvae derived from Ontario field-collections were tested in concentration-response diet-overlay bioassays with lyophilized Cry1F protein at concentrations up to 75 µg cm-2. The concentrations at which mortality of 50% (LC50) of the collections occurred ranged from approximately 10 µg cm-2 (F0) to &gt;28 µg cm-2 (F1) in a 7-d bioassay, indicating relative insensitivity to Cry1F. Results from field experiments, laboratory bioassays, and the history of exposure to Cry1F in corn show that S. albicosta in Ontario are not controlled by Cry1F-expressing corn hybrids and provide evidence for the conclusion that the evolution of resistance to Cry1F has occurred. </p>