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      Genome-Wide Insertional Mutagenesis of Arabidopsis thaliana

      Science

      American Association for the Advancement of Science (AAAS)

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          Abstract

          Over 225,000 independent Agrobacterium transferred DNA (T-DNA) insertion events in the genome of the reference plant Arabidopsis thaliana have been created that represent near saturation of the gene space. The precise locations were determined for more than 88,000 T-DNA insertions, which resulted in the identification of mutations in more than 21,700 of the approximately 29,454 predicted Arabidopsis genes. Genome-wide analysis of the distribution of integration events revealed the existence of a large integration site bias at both the chromosome and gene levels. Insertion mutations were identified in genes that are regulated in response to the plant hormone ethylene.

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          Author and article information

          Journal
          Science
          Science
          American Association for the Advancement of Science (AAAS)
          0036-8075
          1095-9203
          August 01 2003
          August 01 2003
          : 301
          : 5633
          : 653-657
          Article
          10.1126/science.1086391
          12893945
          be225a36-8119-4070-8c0e-9e935e3205bc
          © 2003

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