Over 225,000 independent Agrobacterium transferred DNA (T-DNA) insertion events in
the genome of the reference plant Arabidopsis thaliana have been created that represent
near saturation of the gene space. The precise locations were determined for more
than 88,000 T-DNA insertions, which resulted in the identification of mutations in
more than 21,700 of the approximately 29,454 predicted Arabidopsis genes. Genome-wide
analysis of the distribution of integration events revealed the existence of a large
integration site bias at both the chromosome and gene levels. Insertion mutations
were identified in genes that are regulated in response to the plant hormone ethylene.