Preterm infants are exposed to many stressors within the neonatal intensive care environment. Since these are associated with medical and developmental problems, a reduction of stress factors is desirable. Handling is a very common occurrence and is associated with stress. Kinaesthetic infant handling (KIH) offers practical guidance on handling for nurses and has been widely implemented in Germany, although there is no direct experimental evidence to support its use. The findings of this pilot study suggest a beneficial effect on the SpO2 for infants who were handled using KIH. Since this was a pilot study with a small sample size, caution has to be exercised with this finding. Therefore, the main purpose of the study was: (1) to identify the weaknesses and strengths of the chosen study design; and (2) to perform a power analysis in order to determine a sufficient sample size for the full experiment.