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      Cloning by functional expression of a member of the glutamate receptor family.

      Nature
      Amino Acid Sequence, Animals, Aspartic Acid, analogs & derivatives, pharmacology, Base Sequence, Brain Chemistry, Cloning, Molecular, DNA, genetics, isolation & purification, Electrophysiology, Gene Expression, Glutamates, Glutamic Acid, Ion Channels, drug effects, physiology, Kainic Acid, metabolism, Molecular Sequence Data, Molecular Weight, N-Methylaspartate, Oocytes, Plasmids, RNA, analysis, Rats, Receptors, Glutamate, Receptors, Neurotransmitter, Tissue Distribution, Transcription, Genetic, Transfection, Xenopus

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          Abstract

          We have isolated a complementary DNA clone by screening a rat brain cDNA library for expression of kainate-gated ion channels in Xenopus oocytes. The cDNA encodes a single protein of relative molecular mass (Mr) 99,800 which on expression in oocytes forms a functional ion channel possessing the electrophysiological and pharmacological properties of the kainate subtype of the glutamate receptor family in the mammalian central nervous system.

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