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      Voltage dependence of NMDA-activated macroscopic conductances predicted by single-channel kinetics

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      The Journal of Neuroscience
      Society for Neuroscience

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          Abstract

          The conductance activated in many mammalian CNS neurons by the glutamate analog NMDA is inhibited at hyperpolarized potentials by extracellular magnesium. Whole-cell recordings from hippocampal neurons in culture were used to determine the voltage dependence of the NMDA conductance in the presence of extracellular magnesium concentrations from 1 microM to 10 mM. The conductance-voltage data are well fitted by a gating function derived from rate constants determined in an earlier study of the kinetic behavior of single channels activated by NMDA. The results are consistent with the assumption that magnesium inhibits current through the NMDA-activated channel by directly blocking the ion pore. In addition, another voltage-dependent blocking or flicker- producing mechanism has to be invoked to account for the behavior of the conductance at both the single-channel and whole-cell level, especially at low concentrations of extracellular magnesium.

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          Author and article information

          Journal
          J Neurosci
          J. Neurosci
          jneuro
          The Journal of Neuroscience
          Society for Neuroscience
          0270-6474
          1529-2401
          1 September 1990
          : 10
          : 9
          : 3178-3182
          Affiliations
          Vollum Institute, Oregon Health Sciences University, Portland 97201.
          Article
          PMC6570236 PMC6570236 6570236 jneuro;10/9/3178
          10.1523/JNEUROSCI.10-09-03178.1990
          6570236
          1697902
          be6d86ba-17a2-46d6-9a77-727cddacce60
          © 1990 by Society for Neuroscience
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          10/9/3178
          3178

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