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      • Record: found
      • Abstract: found
      • Article: found

      Specific Radioimmunoassay to Investigate Rod Outer Segment Phagocytosis by Retinal Pigment Epithelium in vitro

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          Abstract

          A sensitive radioimmunoassay has been developed which allows rapid quantitation of rod outer segment (ROS) phagocytosis by retinal pigment epithelial (RPE) explants in vitro. It involves the use of an antiopsin antiserum, in conjunction with <sup>125</sup>I-protein A as a second antibody, and utilizes permeabilization with ethanol to distinguish between the binding and ingestion phases of phagocytosis. This procedure will be used in the future to investigate potential regulatory factors of ROS phagocytosis by retinal pigment epithelium and to evaluate animal models of retinal degeneration.

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          Author and article information

          Journal
          Journal ID (publisher-id): ORE
          Journal ID (nlm-ta): Ophthalmic Res
          Journal ID (doi): 10.1159/issn.0030-3747
          Title: Ophthalmic Research
          Publisher: S. Karger AG
          ISSN (Print): 0030-3747
          ISSN (Electronic): 1423-0259
          Publication date Subscription-year: 1991
          Publication date (Print): 1991
          Publication date (Electronic): 10 December 2009
          Volume: 23
          Issue: 4
          Pages: 171-176
          Affiliations
          aDepartment of Pharmacy, University of Strathclyde, Glasgow; bTennent Institute of Ophthalmology, University of Glasgow, UK
          Article
          Publisher ID: 267100 Other ID: Ophthalmic Res 1991;23:171–176
          DOI: 10.1159/000267100
          PubMed ID: 1834969
          SO-VID: be85e7c7-5069-461c-a492-bba3e066479b
          Copyright © © 1991 S. Karger AG, Basel
          License:

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          History
          Date received : 10 July 1990
          Date accepted : 04 December 1990
          Page count
          Pages: 6
          Categories
          Subject: Paper

          ScienceOpen disciplines: Vision sciences,Ophthalmology & Optometry,Pathology
          Keywords: Retinal pigment epithelium,Phagocytosis,Rod outer segments,Retina,Immunoassay
          Data availability:
          ScienceOpen disciplines: Vision sciences, Ophthalmology & Optometry, Pathology
          Keywords: Retinal pigment epithelium, Phagocytosis, Rod outer segments, Retina, Immunoassay

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