0
views
0
recommends
+1 Recommend
1 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found

      Maternal Stress Decreases Steroid Aromatase Activity in Brains of Male and Female Rat Fetuses

      , ,

      Neuroendocrinology

      S. Karger AG

      Maternal stress, Steroid aromatase, Rat, Fetus, Brain

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Steroid aromatase activity was measured in homogenates of combined hypothalamic and amygdaloid specimens obtained from 17- to 21-day-old male and female rat fetuses. The fetuses were obtained both from normal mothers and mothers exposed to a regimen of stress that results in a failure of behavioral masculinization and defeminization of male offspring. Tissue samples from stressed mothers and controls were obtained during the dark phase of the day (Villanova group, days 17, 18, 19, 20 and 21 postconception). Additional samples were obtained during the light phase (Hershey group, days 17.5, 18.5, 19.5 and 20.5 postconception). The aromatase assay used, based on the measurement of tritiated water formed during an incubation with [lβ-<sup>3</sup>H] androstenedione, had a sensitivity of 10–15 fmol per tube. There was no sex difference in aromatase activity in fetuses of either control or stressed mothers. When data from the two sexes were combined, the following sfatistically significant effects were identified: (1) lower aromatase activity in stressed compared with control fetuses on days 18, 19 and 20 postconception, (2) a progressive decline in enzyme activity between days 18.5 and 20.5 postconception in the Hershey group (controls) and between days 19 and 20 in both the control and stressed fetuses in the Villanova group, and (3) an increase in enzyme activity in the stressed fetuses between days 20 and 21. No relationship was evident between steroid aromatase activity in brain and circulating testosterone levels in male fetuses as determined in a previous study. Failure to detect sex differences or an effect of testosterone on aromatase activity could be due to tissue dilution since enzyme activity may be concentrated in a few discrete nuclear regions. Measurement of enzyme activity in these regions is needed before arriving at any definitive conclusions.

          Related collections

          Author and article information

          Journal
          NEN
          Neuroendocrinology
          10.1159/issn.0028-3835
          Neuroendocrinology
          S. Karger AG
          0028-3835
          1423-0194
          1982
          1982
          26 March 2008
          : 35
          : 5
          : 374-379
          Affiliations
          Division of Reproductive Biology, Department of Obstetrics and Gynecology, The MS Hershey Medical Center, Pennsylvania State University, Hershey, Pa., USA; Department of Psychology, Villanova University, Villanova, Pa., USA
          Article
          123410 Neuroendocrinology 1982;35:374–379
          10.1159/000123410
          7145028
          © 1982 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          Page count
          Pages: 6
          Categories
          Original Paper

          Comments

          Comment on this article