Urinary Transforming Growth Factor-β (TGF-β) Excretion and Renal Production of TGF-β in Rats with Subtotal Renal Ablation: Effect of Enalapril and Nifedipine

Abstract

The aim of the present study was to investigate the effect of enalapril and nifedipine on renal transforming growth factor-β (TGF-β) production and on the rate of urinary TGF-β excretion in rats with subtotal renal ablation. After subtotal nephrectomy some animals were treated with enalapril or nifedipine. Renal cortical TGF-β mRNA levels were 68% higher in untreated nephrectomized rats (p < 0.05) and 39% higher in rats treated with nifedipine (p < 0.05) compared with controls. There was no difference in renal cortical TGF-β mRNA content between the nephrectomized rats treated with enalapril and sham animals, showing that enalapril treatment prevented the increase of TGF-β mRNA in nephrectomized rats. The rate of urinary TGF-β excretion was 2.2 ± 0.8 pg/min in sham animals, 61.5 ± 40.1 pg/min in untreated nephrectomized rats, 9.6 ± 4.2 pg/min in nephrectomized rats treated with enalapril, and 55.2 ± 24.46 pg/min in rats treated with nifedipine. The immunohistochemical reaction for TGF-β in the renal cortex was less intense in the nephrectomized rats treated with enalapril than in the other groups of rats with subtotal renal ablation. These data show that enalapril induces a decrease in renal TGF-β production and in urinary TGF-β excretion in rats with subtotal renal ablation, an effect associated with the protective action of this treatment on renal structure and function and suggest that the determination of the rate of urinary TGF-β could be a useful procedure for the evaluation of disease progression and therapeutic efficacy in the remnant kidney model.

Journal

Journal ID (publisher-id): NEF

Journal ID (nlm-ta): Nephron

Journal ID (doi): 10.1159/issn.1660-8151

Title: Nephron

Publisher: S. Karger AG

ISSN (Print): 1660-8151

ISSN (Electronic): 2235-3186

Publication date Subscription-year: 1998

Publication date (Print): March 1998

Publication date (Electronic): 25 February 1998

Volume: 78

Issue: 3

Pages: 302-309

Affiliations

Departments of ^a Physiology, ^b Pathology and ^c Internal Medicine, Medical School of Ribeirão Prêto, University of São Paulo, Ribeirão Prêto, SP, Brazil

Article

Publisher ID: 44940 Other ID: Nephron 1998;78:302–309

DOI: 10.1159/000044940

PubMed ID: 9546691

Copyright statement: © 1998 S. Karger AG, Basel

License:

Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

Page count

Figures: 5, Tables: 1, References: 42, Pages: 8

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Categories

Subject: Original Paper