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      Distribution of Water-Soluble Crystallins in Microsectioned Cataractous Lenses from One Hundred Egyptian Patients

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          Morphologically classified lenses from 100 cataract patients from the Mansoura Eye Hospital (Egypt) were microdissected and the crystallin composition and distribution were analyzed by thin-layer isoelectric focusing (IEF). The IEF profiles of cataractous lenses were compared with each other, with those of sclerotic lenses and with a normal lens profile. The alterations in the composition of high-molecular-weight (HMW)-, α-, β<sub>H</sub>-, β<sub>L</sub>-, β<sub>S</sub>-, and γ-crystallins along with normal aging, are superimposed by pronounced cataract-related changes which are different for the various types of cataracts. The general feature includes a continuous loss of γ-, β<sub>S</sub>- and β<sub>L</sub>-crystallins of higher IEPs and an increase of HMW material. This is highly pronounced in the nucleus of nuclear cataractous lenses. In cortical cataractous lenses, changes start in the cortical layers. No differences could be observed between alterations in Caucasian lenses and this extended Egyptian lens population.

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          Author and article information

          Ophthalmic Res
          Ophthalmic Research
          S. Karger AG
          11 December 2009
          : 27
          : Suppl 1
          : 54-61
          aInstitute for Experimental Ophthalmology, University of Bonn, Germany; bDepartement of Ophthalmology, College of Medicine, Mansoura University, Mansoura, Egypt; cRadiological Clinic, Experimental Radiology and Radiation Biology, University of Bonn, Germany
          267839 Ophthalmic Res 1995;27:54–61
          © 1995 S. Karger AG, Basel

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          Page count
          Pages: 8
          Contributions to Lens-Morphology, -Physiology, -Biochemistry and -Pharmacology


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