Influence of anterior chamber depth, anterior chamber volume, axial length, and lens density on postoperative endothelial cell loss

Human endothelial morphologic changes were quantitated by specular microscopy and computer-assisted morphometry to establish normal baselines of various morphologic parameters. Cellular polymegethism and cellular pleomorphism increases with age, and normal baseline parameters are detailed. Furthermore, no significant difference in any morphologic parameters between the right and left eye and between central and peripheral endothelium was detected in the normal corneas examined. These normal morphologic baselines can possibly be utilized to detect early corneal endothelial pathology and/or cell loss nondetectable by cell density measurement.

To compare endothelial cell damage during cataract surgery performed using the divide-and-conquer or phaco-chop nuclear fracturing technique. Department of Ophthalmology, Frederiksberg University Hospital, Frederiksberg, Denmark. In a prospective study, 60 eyes of 60 patients were randomly assigned to 1 of 2 groups (30 eyes each group) based on the phacoemulsification technique used: phaco chop or divide and conquer. During surgery, phaco time, phaco power, irrigation volume, and grade of nuclear firmness were recorded. The endothelial cell density, variation in endothelial cell size, percentage of hexagonal cells, and central corneal thickness (CCT) were recorded at baseline and 3 and 12 months postoperatively. Sample size was based on a power calculation (power 0.90, P = .05, clinically important difference = 300 cells/mm2). Significantly less phaco power was used during phaco-chop surgery than during divide-and-conquer surgery. Postoperatively, both groups had a significant but equal decrease in cell density. There were no statistically significant changes in variation in endothelial cell size, percentage of hexagonal cells, or CCT. Visual acuity increased significantly and equally in the 2 groups. Higher endothelial cell loss was significantly correlated with shorter axial length. It has been suggested that the phaco-chop technique is less harmful to the corneal endothelium than the divide-and-conquer technique because it uses less phaco power. The findings did not support this hypothesis as similar and low cell loss was observed in the 2 groups.

To investigate the corneal endothelial cell density and morphology in patients with and without diabetes after phacoemulsification with intraocular lens implantation.