Microbial community in resistant and susceptible Churra sheep infected by <i>Teladorsagia circumcincta</i>

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Abstract

Gastrointestinal nematodes (GIN) are a major threat to health and welfare in small ruminants worldwide. Teladorsagia circumcincta is a nematode that inhabits the abomasum of sheep, especially in temperate regions, causing important economic losses. Given that T. circumcincta and microbiome share the same niche, interactions between them and the host are expected. Although it is known that within a sheep breed there are animals that are more resistant than others to infection by GIN, it is not known if the microbiome influences the phenotype of these animals. Under this condition, 12 sheep were classified according to their cumulative faecal egg count (cFEC) at the end of a first experimental infection, 6 as resistant group (RG) and 6 as susceptible group (SG) to T. circumcincta infection. Then, all sheep were experimentally infected with 70,000 L3 of T. circumcincta and at day 7 days post-infection were euthanized. At necropsy, gastric mucosa and gastric content from abomasum were collected to extract bacterial DNA and sequence V3-V4 region from 16S rRNA gene using Ilumina technology. After bioanalysis performed, results showed that α-diversity and β-diversity remained similar in both groups. However, resistant phenotype sheep showed a higher number of bacteria butyrate-fermenting species as Clostridium sensu stricto 1 (abundance in RG: 1.29% and in SG: 0.069%; p = 0.05), and Turicibacter (abundance in RG: 0.31% and in SG: 0.027%; p = 0.07) in gastric content but also Serratia spp in gastric mucosa (abundance in RG: 0.12% and in SG: 0.041%; p = 0.07). A trend towards a significant negative correlation between cFEC and Clostridium sensu stricto 1 abundance in gastric content was detected (r = − 0.537; p = 0.08). These data suggest that microbiome composition could be another factor associated with the development of the resistant phenotype modifying the interaction with the host and the in last instance affecting the individual risk of infection.

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Author and article information

Contributors

María Martínez-Valladares: mmarva@unileon.es

Journal

Journal ID (nlm-ta): Sci Rep

Journal ID (iso-abbrev): Sci Rep

Title: Scientific Reports

Publisher: Nature Publishing Group UK (London )

ISSN (Electronic): 2045-2322

Publication date (Electronic): 21 October 2022

Publication date PMC-release: 21 October 2022

Publication date Collection: 2022

Volume: 12

Electronic Location Identifier: 17620

Affiliations

[1 ]GRID grid.4807.b, ISNI 0000 0001 2187 3167, Instituto de Ganadería de Montaña, , CSIC-Universidad de León, ; 24346 Grulleros, León, Spain

[2 ]GRID grid.4807.b, ISNI 0000 0001 2187 3167, Departamento de Sanidad Animal, Facultad de Veterinaria, , Universidad de León, ; 24071 León, Spain

[3 ]GRID grid.4807.b, ISNI 0000 0001 2187 3167, Departamento de Ciencias Biomédicas, Facultad de Veterinaria, , Universidad de León, ; 24071 León, Spain

[4 ]GRID grid.4807.b, ISNI 0000 0001 2187 3167, Departamento de Producción Animal, Facultad de Veterinaria, , Universidad de León, ; 24071 León, Spain

Article

Publisher ID: 21058

DOI: 10.1038/s41598-022-21058-x

PMC ID: 9587209

PubMed ID: 36271016

SO-VID: c032ef68-db33-4ef0-bc4d-8e50fe0a4074

License:

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

History

Date received : 18 May 2022

Date accepted : 22 September 2022

Custom metadata

ScienceOpen disciplines: Uncategorized

Keywords: microbial communities,parasitology

Data availability:

ScienceOpen disciplines: Uncategorized

Keywords: microbial communities, parasitology

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Microbial community in resistant and susceptible Churra sheep infected by Teladorsagia circumcincta

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