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      Biochemical Characterization of a Digitalis-Like Substance in Human Urine, Partially Purified by High Pressure Liquid Chromatography

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          Abstract

          We have partially purified a digitalis-like extract from human urine by means of high pressure liquid chromatography (HPLC). Four fractions, giving positive activity for the cross-reaction in the digoxin radioimmunoassay, Na-K-ATPase inhibition or competition with ouabain binding to its receptor, were detected on HPLC. Among these fractions, the first (F-l) was shown to contain the principle fraction, sharing all the activities, and to exhibit quite similar characteristics to ouabain in the modes of Na-K-ATPase inhibition, ouabain displacement activity and positive inotropic action.

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          Author and article information

          Journal
          CRD
          Cardiology
          10.1159/issn.0008-6312
          Cardiology
          S. Karger AG
          0008-6312
          1421-9751
          1989
          1989
          12 November 2008
          : 76
          : 6
          : 442-449
          Affiliations
          Second Department of Internal Medicine, School of Medicine, Kanazawa University, Kanazawa, Japan
          Article
          174531 Cardiology 1989;76:442–449
          10.1159/000174531
          2558793
          c0396c67-2f3b-4878-8151-cfdccd962738
          © 1989 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          History
          : 23 April 1989
          : 24 July 1989
          Page count
          Pages: 8
          Categories
          Original Paper

          General medicine,Neurology,Cardiovascular Medicine,Internal medicine,Nephrology
          Na-K-ATPase inhibition,Ouabain-binding inhibition,Digitalis-like substance

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