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      Disrupting folate metabolism reduces the capacity of bacteria in exponential growth to develop persisters to antibiotics

      1 , 2 , 3 , 4

      Microbiology

      Microbiology Society

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          Most cited references 34

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          Bacterial persistence as a phenotypic switch.

          A fraction of a genetically homogeneous microbial population may survive exposure to stress such as antibiotic treatment. Unlike resistant mutants, cells regrown from such persistent bacteria remain sensitive to the antibiotic. We investigated the persistence of single cells of Escherichia coli with the use of microfluidic devices. Persistence was linked to preexisting heterogeneity in bacterial populations because phenotypic switching occurred between normally growing cells and persister cells having reduced growth rates. Quantitative measurements led to a simple mathematical description of the persistence switch. Inherent heterogeneity of bacterial populations may be important in adaptation to fluctuating environments and in the persistence of bacterial infections.
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            Persister cells and tolerance to antimicrobials.

            Bacterial populations produce persister cells that neither grow nor die in the presence of microbicidal antibiotics. Persisters are largely responsible for high levels of biofilm tolerance to antimicrobials, but virtually nothing was known about their biology. Tolerance of Escherichia coli to ampicillin and ofloxacin was tested at different growth stages to gain insight into the nature of persisters. The number of persisters did not change in lag or early exponential phase, and increased dramatically in mid-exponential phase. Similar dynamics were observed with Pseudomonas aeruginosa (ofloxacin) and Staphylococcus aureus (ciprofloxacin and penicillin). This shows that production of persisters depends on growth stage. Maintaining a culture of E. coli at early exponential phase by reinoculation eliminated persisters. This suggests that persisters are not at a particular stage in the cell cycle, neither are they defective cells nor cells created in response to antibiotics. Our data indicate that persisters are specialized survivor cells.
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              TREATMENT OF STAPHYLOCOCCAL INFECTIONS WITH PENICILLIN BY INTERMITTENT STERILISATION

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                Author and article information

                Journal
                Microbiology
                Microbiology Society
                1350-0872
                1465-2080
                November 01 2018
                November 01 2018
                : 164
                : 11
                : 1432-1445
                Affiliations
                [1 ] 1​Department of Biology, Edge Hill University, St. Helens Road, Ormskirk, Lancashire, L39 4QP, UK
                [2 ] 2​School of Health Sciences, Liverpool Hope University, Hope Park, L16 9JD, Liverpool, UK
                [3 ] 3​Chemical Engineering Department, University of Chester, Thronton Science Park, CH2 4NU, Chester, UK
                [4 ] 4​School of Health Sciences, Liverpool Hope University, Hope Park, L16 9JD, Liverpool, UK
                Article
                10.1099/mic.0.000722
                © 2018

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