Cost-reduction strategies in massive genomics experiments

Abstract

Many modern biology studies require deep, whole-genome sequencing of hundreds to thousands of samples. Although per-sample costs have dramatically decreased, the total budget for such massive genome sequencing constitutes a significant barrier for poorly funded labs. The costly lab tools required for genomics experiments further hinder such studies. Here, we share two strategies for extensively reducing the costs of massive genomics experiments, including miniaturization of the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (reducing the per-sample total costs to ∼ 1/6 of that charged by service providers) and in-lab 3D model-designing of genomics tools. These strategies not only dramatically release funding pressure for labs, but also provide students with additional training in hands-on genomics and 3D-model-designing skills, demonstrating the high potential for their application in genomics experiments and science education.

Journal

Journal ID (publisher-id): MLST

Title: Marine Life Science & Technology

Publisher: Springer (China )

ISSN (Print): 2096-6490

ISSN (Electronic): 2662-1746

Publication date (Print): 01 November 2019

Publication date (Electronic): 05 November 2019

Volume: 1

Issue: 1

Page: xx

Affiliations

¹Institute of Evolution and Marine Biodiversity, KLMME, Ocean University of China, Qingdao 266003, China

Author notes

*Corresponding author: Hongan Long, E-mail: longhongan@ 123456ouc.edu.cn

Haichao Li and Kun Wu contributed equally to this work.

Article

Publisher ID: s42995-019-00013-2

DOI: 10.1007/s42995-019-00013-2

Copyright statement: © 2019 The Author(s)

License:

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 Unported License (CC BY-NC 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. See https://creativecommons.org/licenses/by-nc/4.0/.

Product

Self URI (journal-page): https://www.springer.com/journal/42995

Categories

Subject: Review