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      A colorimetric aptamer biosensor based on cationic polymer and gold nanoparticles for the ultrasensitive detection of thrombin.

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          Abstract

          A colorimetric assay for the ultrasensitive determination of thrombin based on cationic polymer and gold nanoparticles was presented, in which unmodified gold nanoparticles (AuNPs) was used as probes and 21-mer thrombin-binding aptamer (TBA) as sensing elements. Upon the addition of thrombin, TBA interacted specifically with thrombin to form a G-quadruplex structure. As a result, the conformation change facilitated the cationic polymer, poly(diallyldimethylammonium chloride) (PDDA)-induced AuNP aggregation. Thus, the visible change in color from wine-red to blue-purple was readily seen by the naked eye. The colorimetric sensor could detect thrombin down to 1 pM with high selectivity in the presence of other interferring proteins. Furthermore, the assay was successfully employed to determine thrombin in human serum sample, which suggested its great potential for diagnostic purposes.

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          Author and article information

          Journal
          Biosens Bioelectron
          Biosensors & bioelectronics
          1873-4235
          0956-5663
          Jun 15 2014
          : 56
          Affiliations
          [1 ] Department of Chemistry, Capital Normal University, Beijing 100048, China. Electronic address: czb979216@sina.com.
          [2 ] Department of Chemistry, Capital Normal University, Beijing 100048, China.
          Article
          S0956-5663(14)00015-3
          10.1016/j.bios.2014.01.012
          24463195
          c14890ba-f4a2-4481-a7e6-7450d4aaaa68
          Copyright © 2014 Elsevier B.V. All rights reserved.
          History

          Analytical chemistry
          Thrombin,Gold nanoparticles,Colorimetry,Cationic polymer,Aptamer
          Analytical chemistry
          Thrombin, Gold nanoparticles, Colorimetry, Cationic polymer, Aptamer

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