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      In vitro Release of Prolactin by Thyrotropin-Releasing Hormone: Influence of Dopamine, Thyroxine, and Cycloheximide

      research-article
      ,
      Hormone Research in Paediatrics
      S. Karger AG
      Prolactin, Thyrotropin-releasing hormone, Thyrotropin, Dopamine, Thyroxine, Cycloheximide

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          Abstract

          An acute incubation procedure, using explanted normal rat hemipituitaries pretreated with fresh plasma obtained from pituitary donor animals, was employed to further investigate the in vitro stimulation of prolactin (PRL) release by thyrotropin-releasing hormone (TRH). Pretreatment with dopamine (0.1 µg/ml) caused a 30-50% decrease in the amount of PRL released into incubation media; the inhibitory effect of dopamine was not reversed by treatment with 0.5-6.0 ng. TRH, although these TRH concentrations consistently stimulated PRL release from pituitaries not exposed to dopamine. Treatment with thyroxine (10<sup>-6</sup> to 10<sup>-5</sup> M) showed a competitive inhibition of thyrotropin release by TRH (0.5 ng), but was without effect on TRH-stimulated PRL release. Cycloheximide (100 µg/ml) blocked a net increase in PRL levels. TRH, nevertheless, significantly increased PRL release in the presence of cycloheximide. The results indicate that neither dopamine nor thyroxine compete with TRH in causing PRL release, and that the TRH stimulation of PRL release is unrelated to ongoing levels of hormone synthesis.

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          Author and article information

          Journal
          HRE
          Horm Res Paediatr
          10.1159/issn.1663-2818
          Hormone Research in Paediatrics
          S. Karger AG
          1663-2818
          1663-2826
          1979
          1979
          25 November 2008
          : 11
          : 3
          : 128-141
          Affiliations
          Department of Anatomy, University of Rochester School of Medicine and Dentistry, Rochester, N.Y.
          Article
          179048 Horm Res 1979;11:128–141
          10.1159/000179048
          114468
          c19e385b-626c-46a6-9fbd-fe9a95835c23
          © 1979 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          History
          : 24 July 1978
          : 05 February 1979
          Page count
          Pages: 14
          Categories
          Original Paper

          Endocrinology & Diabetes,Neurology,Nutrition & Dietetics,Sexual medicine,Internal medicine,Pharmacology & Pharmaceutical medicine
          Thyrotropin,Prolactin,Cycloheximide,Thyroxine,Thyrotropin-releasing hormone,Dopamine

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