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CRISPR-Based Technologies for the Manipulation of Eukaryotic Genomes
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Abstract
The CRISPR-Cas9 RNA-guided DNA endonuclease has contributed to an explosion of advances
in the life sciences that have grown from the ability to edit genomes within living
cells. In this Review, we summarize CRISPR-based technologies that enable mammalian
genome editing and their various applications. We describe recent developments that
extend the generality, DNA specificity, product selectivity, and fundamental capabilities
of natural CRISPR systems, and we highlight some of the remarkable advancements in
basic research, biotechnology, and therapeutics science that these developments have
facilitated.