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      Producción de aloína en callos y hojas de brotes de zábila (Aloe vera L.) regenerados in vitro Translated title: Aloin production in calli and leaves of in vitro regenerated shoots of aloe (Aloe vera L.)

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          Aloe vera L. es actualmente una de las plantas de mayor importancia económica y medicinal debido a los metabolitos secundarios que produce, tales como la aloína, que es utilizada en la industria cosmética y farmacéutica. En este trabajo se cuantificó la producción de este compuesto en callos y hojas de brotes regenerados in vitro, cultivados durante 3, 6 y 12 meses, comparándolos con la producción en medios controles y plantas silvestres de esta especie. Se utilizó medio Murashige y Skoog (MS) suplementado con diferentes concentraciones hormonales. La edad del cultivo fue factor importante en la producción de aloína en el material estudiado (hojas de brotes regenerados in vitro). Se observó que la producción de aloína fue muy variable, siendo mayor en callos basales cultivados en el medio que contenía 0,5 mg·L-1 de 2,4-D y 0,1 mg·L-1 de cinetina, con resultados superiores a los encontrados en los medios controles y en hojas de plantas silvestres. El contenido de aloína en hojas de brotes regenerados in vitro cultivados en medio MS con 0,5 mg·L-1 de 2,4-D y 5 mg·L-1 de cinetina, fue menor al de los callos pero levemente superior al de plantas silvestres. Estos resultados muestran que los cultivos de callos son una fuente importante para la producción de metabolitos secundarios en zábila.

          Translated abstract

          Aloe vera L. is currently an important commercial and medicinal plant because it produces secondary metabolites, such as aloin, which is used in cosmetics and pharmaceuticals. In this research, we quantified the production of this compound in calluses and leaves of in vitro regenerated shoots, cultured for 3, 6 and 12 months, compared with the content in controls and wild plants. The Murashige and Skoog medium (MS) supplemented with different hormone levels was used. The age of the cultures was an important factor for aloin production in the studied material (leaves of in vitro regenerated shoots). The aloin production was variable, being higher in basal callus grown on medium containing 2,4-D (0.5 mg·L-1) and kinetin (0.1 mg·L-1), and higher than the production observed in control media and wild plant leaves. The aloin content in leaves of in vitro regenerated shoots cultured in MS supplemented with 2,4-D (0.5 mg.L-1) and kinetin (5 mg·L-1) was lower than that in the callus but slightly higher than in wild plants. These results show that callus cultures represent a promising source for secondary metabolites production in A. vera.

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          Most cited references 33

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          Production of plant secondary metabolites: a historical perspective

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            Protective effect of Aloe vera on polymicrobial sepsis in mice.

            Sepsis is an acute life-threatening clinical condition and remains the major cause of death in intensive care units. The primary pathophysiologic event central to the septic response is an overwhelming activation of the inflammatory system and countervailing response from the anti-inflammatory system. However, the cause of this perturbation has yet to be elucidated. In this study, we report that Aloe vera therapeutically reverses the lethality induced by cecal ligation and puncture (CLP), a clinically relevant model of sepsis. The administration of Aloe vera ameliorated the multiple organ dysfunction syndrome, as evidenced by the serum levels of biochemical parameters and histological changes. In order to investigate the pharmacological mechanism of Aloe vera, the levels of the cytokines, tumor necrosis factor (TNF)-alpha, interleukin (IL)-1beta, and IL-6 were determined by ELISA at various time points. The increases in the levels of TNF-alpha, IL-1beta, and IL-6 were attenuated by Aloe vera.In vivo administration of Aloe vera also markedly enhanced bacterial clearance. Our findings suggest that Aloe vera could be a potential therapeutic agent for the clinical treatment of sepsis.
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              Taxol content in the bark of Himalayan Yew in relation to tree age and sex.

               M. Nadeem (2002)
              Taxol content in the bark of Taxus baccata trees growing in a homogenous (uniform) environment at Jageshwar, District Almora in Central Himalaya has been quantified. The average taxol concentration in the bark of sampled trees was 0.0558+/-0.008% (of dry wt.) and was about 64% higher for male plants (averaged across tree age) in comparison to female trees. Maximum taxol content was recorded in the bark samples collected from trees of >110 yrs age. ANOVA indicates a significant difference in the taxol content of bark from trees of different ages, however, differences were not significant between sexes. Taxol was quantified by HPLC using a standard curve prepared with authentic taxol; the identification of bark taxol was confirmed by UV and mass spectrometry. The total taxol content of the bark of Taxus trees across an age series was found to range between 0.064 to 8.032 g/tree, and a tree of about 100 yrs age can yield 5.74 kg dry bark.

                Author and article information

                Role: ND
                Decanato de Agronomía de la Universidad Centroccidental "Lisandro Alvarado" (UCLA) (Barquisimeto )
                August 2011
                : 23
                : 2
                : 79-86
                [1 ] Universidad del Zulia Venezuela
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