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      A novel geminivirus identified in tomato and cleome plants sampled in Brazil.

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          Abstract

          Viruses in the family Geminiviridae have single-stranded DNA genomes encapsulated in geminate icosahedral particles. High throughput sequencing (HTS) for metagenomic approaches are being extensively used for the identification of known and novel viruses. Using a HTS approach, we identified a novel geminivirus in a tomato (Solanum lycopersicum) sample and a Cleome sp. sample collected in the midwest region of Brazil. The genomes from the two samples share 99.96% identity and ∼61-63% to genomes in the genus Capulavirus. The novel virus has been tentatively named tomato associated geminivirus 1 (TaGV1). No visual symptoms were observed in the field tomato plant or in the inoculated Nicotiana benthamiana where the virus established a systemic infection. The replication associated protein of TaGV1 is most similar to that encoded by capulaviruses (sharing 62-70% identity), whereas the CP is most similar to that of tomato pseudo curly top virus (sharing ∼31% identity). In the TaGV1 positive Cleome sp. sample, begomovirus DNA A and B components were also detected sharing 96% and 90% sequence identity to cleome leaf crumple virus DNA A and B components, respectively. Using a HTS approach, we identified TaGV1 in tomato and Cleome sp. samples and this is the first report of a geminivirus that is non-begomovirus in Brazil.

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          Author and article information

          Journal
          Virus Res.
          Virus research
          Elsevier BV
          1872-7492
          0168-1702
          August 15 2017
          : 240
          Affiliations
          [1 ] Embrapa Recursos Genéticos e Biotecnologia, Brasília, Brazil; The Biodesign Center for Fundamental and Applied Microbiomics, Center for Evolution and Medicine, School of Life Sciences, Arizona State University, Tempe, AZ, 85287, USA.
          [2 ] Embrapa Recursos Genéticos e Biotecnologia, Brasília, Brazil.
          [3 ] The Biodesign Center for Fundamental and Applied Microbiomics, Center for Evolution and Medicine, School of Life Sciences, Arizona State University, Tempe, AZ, 85287, USA; Structural Biology Research Unit, Department of Clinical Laboratory Sciences, University of Cape Town, Observatory, Cape Town, South Africa. Electronic address: arvind.varsani@asu.edu.
          [4 ] Embrapa Recursos Genéticos e Biotecnologia, Brasília, Brazil. Electronic address: simone.ribeiro@embrapa.br.
          Article
          S0168-1702(17)30438-0
          10.1016/j.virusres.2017.08.007
          28843502
          c1b378fc-2d69-4e2e-9c72-1885d37a91d0
          History

          Cleome sp,Geminivirdae,Solanum lycopersicum
          Cleome sp, Geminivirdae, Solanum lycopersicum

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