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      Processing of gene expression data generated by quantitative real-time RT-PCR.

      1 , , ,
      BioTechniques

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          Abstract

          Quantitative real-time PCR represents a highly sensitive and powerful technique for the quantitation of nucleic acids. It has a tremendous potential for the high-throughput analysis of gene expression in research and routine diagnostics. However, the major hurdle is not the practical performance of the experiments themselves but rather the efficient evaluation and the mathematical and statistical analysis of the enormous amount of data gained by this technology, as these functions are not included in the software provided by the manufacturers of the detection systems. In this work, we focus on the mathematical evaluation and analysis of the data generated by quantitative real-time PCR, the calculation of the final results, the propagation of experimental variation of the measured values to the final results, and the statistical analysis. We developed a Microsoft Excel-based software application coded in Visual Basic for Applications, called Q-Gene, which addresses these points. Q-Gene manages and expedites the planning, performance, and evaluation of quantitative real-time PCR experiments, as well as the mathematical and statistical analysis, storage, and graphical presentation of the data. The Q-Gene software application is a tool to cope with complex quantitative real-time PCR experiments at a high-throughput scale and considerably expedites and rationalizes the experimental setup, data analysis, and data management while ensuring highest reproducibility.

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          Author and article information

          Journal
          Biotechniques
          BioTechniques
          0736-6205
          0736-6205
          Jun 2002
          : 32
          : 6
          Affiliations
          [1 ] Research Group Cardiovascular Genetics, Institute of Biochemistry and Genetics, University of Basel, Switzerland. patrick.muller@unibas.ch
          Article
          12074169
          c1c33312-3a11-4ec6-9e01-2e84bc947fef
          History

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