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      A Rapid Method for Isolation of Retinal Pigment Epithelial Cells from Rat Eyeballs

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          Abstract

          A new, simple method for isolating retinal pigment epithelial (RPE) cells from rat eyeballs was developed: incubation of the eyeballs in 0.1% proteinase K solution. This method yielded an average of 4 × 10<sup>4</sup> viable RPE cells from each Sprague-Dawley rat eyeball in 1 h. These RPE cells were hexagonal and had cytokeratin in their cytoplasm and numerous microvilli on their surface, similar to RPE cells evaluated in vivo. The results of this study thus show that our method for incubating eyeballs in proteinase K can provide good-quality RPE cells in sufficient quantities for study in a short time.

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          Author and article information

          Journal
          ORE
          Ophthalmic Res
          10.1159/issn.0030-3747
          Ophthalmic Research
          S. Karger AG
          0030-3747
          1423-0259
          1995
          1995
          11 December 2009
          : 27
          : 5
          : 262-267
          Affiliations
          aDepartment of Ophthalmology, Ehime University School of Medicine, Ehime, bSenju Pharmaceutical Co.Ltd., cDepartment of Ophthalmology, Osaka University Medical School, dDivision of Ophthalmology, Osaka Prefectural Habikino Hospital, Osaka, Japan
          Article
          267735 Ophthalmic Res 1995;27:262–267
          10.1159/000267735
          8552366
          c1f868e4-96d1-47f2-ba94-61895cf51bb4
          © 1995 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          History
          : 08 February 1995
          : 27 April 1995
          Page count
          Pages: 6
          Categories
          Original Paper

          Vision sciences,Ophthalmology & Optometry,Pathology
          Retinal pigment epithelium,Proteinase K,Isolation,Rat

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