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      Assaying mRNA deadenylation in vivo.

      1 ,  
      Methods in molecular biology (Clifton, N.J.)

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          Abstract

          Deadenylation is the removal of poly(A) tails from mRNA. Here, we present two methods for assaying deadenylation in vivo. The first is a method for measuring bulk poly(A) tail lengths. When combined with a block in transcription, the method can be used for measuring the rate of bulk poly(A) tail shortening. The second is an RT-PCR method to determine the poly(A) tail lengths of individual RNAs. Again in combination with a block of transcription, the method permits the rate of deadenylation of an individual RNA to be measured.

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          Author and article information

          Journal
          Methods Mol. Biol.
          Methods in molecular biology (Clifton, N.J.)
          1940-6029
          1064-3745
          2014
          : 1125
          Affiliations
          [1 ] Institute of Biochemistry and Biotechnology, Martin-Luther-University Halle-Wittenberg, Kurt-Mothes-Strasse 3, 06120, Halle, Germany.
          Article
          10.1007/978-1-62703-971-0_25
          24590799
          c1fcb09f-bca0-4600-8782-6ca5d352bc00
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