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      The HTLV-1 hbz antisense gene indirectly promotes tax expression via down-regulation of p30-II mRNA

      1 , 1 , , 1

      Retrovirology

      BioMed Central

      15th International Conference on Human Retroviruses: HTLV and Related Viruses

      5-8 June 2011

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          Abstract

          Human T-cell leukemia virus type 1 (HTLV-1) basic leucine zipper factor (HBZ) is transcribed from the antisense genomic DNA strand and functions differently in its RNA and protein forms. To distinguish between the roles of hbz mRNA and HBZ protein, we generated mutants in a proviral clone that specifically disrupt the hbz gene product. A proviral clone with a splice acceptor mutation that disrupts expression of the predominant hbz mRNA resulted in lower levels of tax mRNA. Heterologous hbz expression restored Tax activity in cells expressing this mutant clone. In contrast, proviral mutants that disrupt only HBZ protein did not affect levels of tax mRNA. Expression of hbz resulted in lower levels of p30II mRNA. Mutation of p30II overcame the effects of the splice acceptor mutation of hbz, and restored tax expression. Thus, there is a complex interplay of viral regulatory proteins controlling levels of HTLV-1 gene expression. The pathogenic role of HBZ alone or combined with Tax is currently under study in transgenic mice, and will be discussed.

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          Author and article information

          Conference
          Retrovirology
          Retrovirology
          BioMed Central
          1742-4690
          2011
          6 June 2011
          : 8
          : Suppl 1
          : A136
          Affiliations
          [1 ]Division of Molecular Oncology, St Louis, MO, 63110, USA
          Article
          1742-4690-8-S1-A136
          10.1186/1742-4690-8-S1-A136
          3112605
          Copyright ©2011 Choudhary et al; licensee BioMed Central Ltd.

          This is an open access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

          15th International Conference on Human Retroviruses: HTLV and Related Viruses
          Leuven and Gembloux, Belgium
          5-8 June 2011
          Categories
          Meeting Abstract

          Microbiology & Virology

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