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      Host antitumor resistance improved by the macrophage polarization in a chimera model of patients with HCC.

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          Abstract

          Despite major advances in curative and palliative approaches, hepatocellular carcinoma (HCC) is still the third leading cause of cancer-related death worldwide. M1 macrophages (Mϕ) play a key role in host antitumor defenses in HCC. In our study, CD14+ cells were isolated from the peripheral blood of four groups of HCC patients (group-1, patients with stage 0 HCC; group-2, patients with stage A HCC; group-3, patients with stage B HCC; and group-4, patients with stage C HCC) and characterized phenotypically. Then, CD14+ cells from group-2 and group-3 HCC patients were induced to polarize and tested for their antitumor abilities in a chimera model of HCC patients. Human HCCs (HepG2 solid tumors) grew in a chimera model of group-3 patients (group-3 HCC chimeras) but not in a chimera model of group-2 patients (group-2 HCC chimeras). In response to HCC antigens, the majority of CD14+ cells from group-2 patients (group-2 CD14+ cells) switched to the M1 phenotype (IL-12+IL-10-iNOS+cells), whereas the majority of CD14+ cells from group-3 patients (group-3 CD14+ cells) did not switch to the M1 phenotype and continued to express M2b phenotypic properties (IL-12-IL-10+CCL1+iNOS-cells). Group-3 CD14+ cells showed M1Mϕ polarization after treatment with CCL1 antisense oligodeoxynucleotide (ODN). Therefore, our study indicates that anti-HCC defenses of group-3 HCC chimeras are improved after CCL1 antisense ODN treatment.

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          Author and article information

          Journal
          Oncoimmunology
          Oncoimmunology
          Informa UK Limited
          2162-4011
          2162-4011
          2017
          : 6
          : 4
          Affiliations
          [1 ] Second Department of Internal Medicine, Osaka Medical College, Takatsuki, Japan.
          [2 ] Medical Laboratory, Osaka Medical College, Takatsuki, Japan.
          [3 ] Department of Internal Medicine, The University of Texas Medical Branch, Galveston, TX, USA.
          Article
          1299301
          10.1080/2162402X.2017.1299301
          5414886
          28507807

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