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Regioselectivity of new bacterial lipases determined by hydrolysis of triolein.

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Current microbiology

Springer Nature America, Inc

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      Abstract

      The newly identified lipases of 67 bacterial strains, primarily Bacillus and Pseudomonas, from the ARS Culture Collection have been characterized on the basis of their positional specificity for triglycerides (triolein). Lipase was produced by growing the cultures in tryptone-glucose-yeast extract medium for 24 h at 30 degrees C before addition of triglyceride. The lipase was allowed to act on the triglyceride for 3 days before analysis by thin-layer chromatography. Of the bacterial lipases tested, 55 displayed random specificity, 9 were 1,3-specific, and 3 showed no apparent lipase activity under these conditions.

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      Affiliations
      [1 ] Microbial Genomics and Bioprocessing Research Unit, National Center for Agricultural Utilization Research, ARS, USDA, 1815 North University Street, Peoria, IL 61604-3999, USA. ocacl@mail.ncaur.usda.gov
      Journal
      Curr. Microbiol.
      Current microbiology
      Springer Nature America, Inc
      0343-8651
      0343-8651
      May 2002
      : 44
      : 5
      11927984 10.1007/s00284-001-0019-3

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