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      Regioselectivity of new bacterial lipases determined by hydrolysis of triolein.

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      Current microbiology

      Springer Nature America, Inc

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          Abstract

          The newly identified lipases of 67 bacterial strains, primarily Bacillus and Pseudomonas, from the ARS Culture Collection have been characterized on the basis of their positional specificity for triglycerides (triolein). Lipase was produced by growing the cultures in tryptone-glucose-yeast extract medium for 24 h at 30 degrees C before addition of triglyceride. The lipase was allowed to act on the triglyceride for 3 days before analysis by thin-layer chromatography. Of the bacterial lipases tested, 55 displayed random specificity, 9 were 1,3-specific, and 3 showed no apparent lipase activity under these conditions.

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          Author and article information

          Affiliations
          [1 ] Microbial Genomics and Bioprocessing Research Unit, National Center for Agricultural Utilization Research, ARS, USDA, 1815 North University Street, Peoria, IL 61604-3999, USA. ocacl@mail.ncaur.usda.gov
          Journal
          Curr. Microbiol.
          Current microbiology
          Springer Nature America, Inc
          0343-8651
          0343-8651
          May 2002
          : 44
          : 5
          10.1007/s00284-001-0019-3
          11927984

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